Log In Sign up
The largest database of trusted experimental protocols

Anti p jnk sc 6254

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

Anti-p-JNK (sc-6254) is a polyclonal antibody that recognizes the phosphorylated form of c-Jun N-terminal kinase (JNK). JNK is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in cellular responses to various stimuli.

Automatically generated - may contain errors

Lab products found in correlation

Metaphosphoric acid, by Merck Group (1 mentions) Anti β actin sc 69879, by Santa Cruz Biotechnology (1 mentions) Superoxide dismutase sod kit, by Dojindo Laboratories (1 mentions) Lps escherichia coli o55 b5, by Merck Group (1 mentions) Hepes, by Merck Group (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Sodium bicarbonate, by Merck Group (1 mentions) Eclipse 80i light microscope, by Nikon (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti p p38 sc 7973, by Santa Cruz Biotechnology (1 mentions) Anti ub sc 8017, by Santa Cruz Biotechnology (1 mentions) Anti p ampk thr172, by Cell Signaling Technology (1 mentions) Anti cleaved parp, by Cell Signaling Technology (1 mentions) Anti ha tag, by Cell Signaling Technology (1 mentions) Anti p erk1 2, by Cell Signaling Technology (1 mentions) Anti β actin, by Proteintech (1 mentions) Anti akt, by Proteintech (1 mentions) Anti jnk, by Proteintech (1 mentions) Anti flag tag, by Proteintech (1 mentions) Anti nrf2, by Proteintech (1 mentions)

4 protocols using anti p jnk sc 6254

1

Inflammatory Response Regulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
De Man-Rogosa-Sharp (MRS) broth and fructo-oligosaccharide (FOS) were purchased from Difco (USA) and Wako (Japan), respectively. A superoxide dismutase (SOD) kit was obtained from Dojindo Molecular Technologies (USA). Anti-p-JNK (sc-6254) and anti-β-actin (sc-69879) were purchased from Santa Cruz Biotechnology (USA). Anti-p-NF-κB (3033S) and anti-TNF-α (3707S) and secondary antibodies were purchased from Cell Signaling Technology (USA). Anti-IL-6 (P05231) was obtained from Cusabio Biotech (China). A mouse IL-6 enzyme-linked immunosorbent assay (ELISA) kit was purchased from Koma Biotech (Korea). All other products including LPS (Escherichia coli O55: B5), RPMI 1640 medium, HEPES, metaphosphoric acid and sodium bicarbonate were purchased from Sigma-Aldrich Chemical Co. (USA).
Kang J.E., Park S.K., Kang J.Y., Kim J.M., Kwon B.S., Park S.H., Lee C.J., Yoo S.K, & Heo H.J. (2020). Actinidia arguta Sprout as a Natural Antioxidant: Ameliorating Effect on Lipopolysaccharide-Induced Cognitive Impairment. Journal of Microbiology and Biotechnology, 31(1), 51-62.
+ Open protocol
+ Expand
2

Immunofluorescence Analysis of Apoptotic Markers

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The nerve sections were dewaxed, antigen repaired and blocked with 3% BSA. One hour later, sections were incubated in the following primary antibodies overnight at 4 °C: anti-IRE1α (sc-390960; 1: 50; Santa Cruz), anti-p-JNK (sc-6254; 1: 50; Santa Cruz) and rabbit anti-Caspase-12 (sc-5627; 1: 50; Santa Cruz). After rinsing, the sections were incubated with the fluorescein (FITC)-conjugated goat anti-mouse IgG (H+L) or FITC-conjugated goat anti-rabbit IgG (H+L) for 1 h at room temperature. Microphotographs were captured using the Nikon Eclipse 80i light microscope7 (link).
Yao W., Yang X., Zhu J., Gao B., Shi H, & Xu L. (2018). IRE1α siRNA relieves endoplasmic reticulum stress-induced apoptosis and alleviates diabetic peripheral neuropathy in vivo and in vitro. Scientific Reports, 8, 2579.
+ Open protocol
+ Expand
3

Western Blot Analysis of Cellular Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein separation and western blotting were performed as described previously [16 (link)]. Western blot analysis was performed using anti-cleaved PARP (#5625), anti-cleaved caspase-3 (#9661), anti-p-AMPK (Thr172) (#50081), anti-p-ERK1/2 (#4370), and anti-HA-Tag (#3724) antibodies purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-LC3 (14600–1-AP), anti-P62/SQSTM1 (18420–1-AP), anti-phospho-Akt (Ser-473) (66444–1-Ig), anti-Akt (10176–2-AP), anti-mTOR (20657–1-AP), anti-P38 MAPK (14064–1-AP), anti-JNK (51151–1-AP), anti-ERK1/2 (16443–1-AP), anti-AMPK (10929–2-AP), anti-Nrf2 (16396–1-AP), anti-Keap1 (10503–2-AP), anti-FLAG-tag (66008–2-AP), anti-MYC-tag (60003–2-AP), and anti-β-actin (66009–1-Ig) antibodies were obtained from Proteintech Group Co., Ltd. (Wuhan, China). An anti-phospho-mTOR (Ser-2448) (ab109268) antibody was purchased from Abcam (Cambridge, MA, USA). Anti-p-p38 (sc-7973), anti-p-JNK (sc-6254), and anti-Ub (sc-8017) antibodies were obtained from Santa Cruz Biotechnology., Inc. (Santa Cruz, CA, USA).
Liu J.Z., Hu Y.L., Feng Y., Jiang Y., Guo Y.B., Liu Y.F., Chen X., Yang J.L., Chen Y.Y., Mao Q.S, & Xue W.J. (2020). BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer. Journal of Experimental & Clinical Cancer Research : CR, 39, 123.
+ Open protocol
+ Expand
4

Western Blot Analysis of Microglia

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot assay was carried out as the previously described methods (Roman et al., 2020 (link)). Primary microglia and mouse microglia BV2 cells with different treatments were collected, and the total proteins were isolated using RIPA buffer (Gibco, United States). Then, the different protein samples were loaded into the lane of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, followed by the transfer into polyvinylidene fluoride (PVDF) membranes (Sigma-Aldrich, United States). Next, the membranes were placed in 5% skim milk and blocked at RT for 1 h and then incubated with the specific primary antibodies at 4°C overnight. The antibodies were: anti-CD86 (ab243887, 1:1000, Abcam), anti-CD206 (ab252921, 1:1000, Abcam), anti-p-JNK (sc-6254, 1:500, Santa Cruz Biotechnology), anti-JNK(sc-7345, 1:500, Santa Cruz Biotechnology), anti-p-p38 (sc-166182, 1:500, Santa Cruz Biotechnology), anti-p38 (ab170099, 1:2000, Abcam), anti-pERK (sc-7383, 1:500, Santa Cruz Biotechnology), anti-ERK (ab32537, 1:1000, Abcam) and β-actin (ab6276, 1:5000, Abcam). The samples were further incubated with the secondary antibody (ab205718, 1:2000, Abcam) at 37°C for nearly 1 h. Ultimately, the enhanced chemiluminescence reagents (Millipore, Bedford, MA) and ImageJ were applied to analyze protein bands.
Wu L., Zhan Q., Liu P., Zheng H., Liu M., Min J., Xie L, & Wu W. (2022). LncRNA TCONS_00145741 Knockdown Prevents Thrombin-Induced M1 Differentiation of Microglia in Intracerebral Hemorrhage by Enhancing the Interaction Between DUSP6 and JNK. Frontiers in Cell and Developmental Biology, 9, 684842.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!