Vacuette z serum separator clot activator

This study was performed between February and June 2022. Blood samples used in this study were obtained from outpatients referred to the laboratory for blood testing. Samples were drawn in gel separator tubes without anticoagulant (Vacuette Z serum separator clot activator, Greiner Bio-One, Kremsmünster, Austria). After laboratory testing, the remaining serum samples were used for study purposes. No additional blood sampling was needed for this research. Patients data were kept anonymous.
The biochemical analytes studied were CREA, CK, CHOL, GGT, HDL and TP.
This study has been designed employing a study procedure defined by the Spanish Society of Laboratory Medicine (SEQC-ML), which is, in turn, based on CLSI document EP7-A2 (1 , 17 ). The Hospital’s ethics committee approved the study as clinical research.

Peripheral blood and urine samples were obtained from patients between 2 and 65 year old with suspected arbovirus infection, who presented with fever, rash, and/or arthralgia symptoms. Venous whole blood samples were collected in one VACUETTE^® Z Serum Separator Clot Activator and two Vacuette® EDTA Tubes (Greiner Bio-One, Kremsmünster, Austria). To one EDTA tube, RNAlater (Thermo Fisher Scientific) was added at half the volume of the collected blood samples to prevent RNA degradation during transport. In total, 90 plasma/serum and 99 urine samples from 120 patients with suspected arbovirus infection, including paired samples from 69 cases, were used in this study. The separated plasma or serum samples and urine samples were stored at −80 °C until use. RNAs were extracted from sera and urine using the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s instructions. RNA samples were eluted with 60 µL of elution buffer and stored at −80 °C until use.