2 3 cgamp tlrl nacga23 02

Manufactured by InvivoGen

2'3'-cGAMP (tlrl-nacga23-02) is a synthetic cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) analogue. It is a molecular agonist of the stimulator of interferon genes (STING) pathway.

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2′3′-cGAMP (127 citations) CGAMP (67 citations) 3′3′-cGAMP (16 citations) Tlrl-nacga23 (15 citations) 2’,3’-cGAMP (tlrl-nacga23) (2 citations) Tlrl-nacga23-02 (2 citations)

2 protocols using 2 3 cgamp tlrl nacga23 02

STING Pathway Activation and Inhibition

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2′3′-cGAMP (tlrl-nacga23-02) was purchased from InvivoGen. Ibrutinib (BTK inhibitor; no. S2680), PF-562271 (FAK inhibitor; no. S2890), PP2 (Src inhibitor; no. S7008), saracatinib (Fyn inhibitor; no. S1006), amuvatinib (c-Kit inhibitor; no. S1244), ruxolitinib (Jak1/2 inhibitor; no. S1378), alectinib (ALK inhibitor; no. S2762), gefitinib (EGFR inhibitor; no. S1025), AZD9291 (EGFR inhibitor; no. S7297), and R406 (Syk inhibitor; no. S2194) were obtained from Selleck. Polyethyleneimine (PEI; no. 195444) was purchased from Mpbio, and the transfection procedure was as previously described (16 (link), 33 (link)). Lipofectamine 2000 (no. 11668019) was obtained from Invitrogen. Antibodies against EGFR (no. 4267), GFP (no. 2956), phospho-Syk (Tyr525/526) (no. 2710), Syk (no. 13198), tubulin (no. 2146), and actin (no. 3700) were from Cell Signaling, and hemagglutinin (HA) antibody (ab18181) was purchased from Abcam. STING antibody (A3575) and GST (AE001) antibodies were obtained from Abclonal Technology.

Wang C., Sharma N., Veleeparambil M., Kessler P.M., Willard B, & Sen G.C. (2021). STING-Mediated Interferon Induction by Herpes Simplex Virus 1 Requires the Protein Tyrosine Kinase Syk. mBio, 12(6), e03228-21.

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Immune Cell Activation Assays

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For in vitro studies, we used LPS (ALX-581–013-L002) from ENZO, Poly (I:C) HMW (tlr-pic), R848 (tlr-r848), CpG(C) (tlrl-2395), 2’3’cGAMP (tlrl-nacga23–02) and 3p-hpRNA/LyoVec (tlrl-hprnalv) purchased from Invivogen. Lipofectamine 3000 Transfection Reagent (L3000–008) was purchased from Invitrogen. Phagocytes were cultured in RPMI 1640 medium + GlutaMAX (72400-047) with 1% Penicillin-Streptomycin (15140122) purchased from Thermo Fisher, and 10% fetal bovine serum (FBS). HBECs were cultured as previously described (Major et al., 2020). For flow cytometry we used PerCP/Cy5.5 CD14 (clone HCD14), APC/Cyanine7 HLA-DR (clone L243), PE/Cy7 CD11c (clone 3.9) and PE CD141 (clone M80) antibodies purchased from Biolegend.

Sposito B., Broggi A., Pandolfi L., Crotta S., Ferrarese R., Sisti S., Clementi N., Ambrosi A., Liu E., Frangipane V., Saracino L., Marongiu L., Facchini F.A., Bottazzi A., Fossali T., Colombo R., Clementi M., Tagliabue E., Pontiroli A.E., Meloni F., Wack A., Mancini N, & Zanoni I. (2021). Severity of SARS-CoV-2 infection as a function of the interferon landscape across the respiratory tract of COVID-19 patients.. bioRxiv.

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