Opal 7 colors manual ihc kit

Manufactured by PerkinElmer

The Opal 7-colors manual IHC kit is a laboratory equipment product designed for manual immunohistochemistry (IHC) applications. It allows for the simultaneous detection of up to 7 different protein targets within a single tissue sample.

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Lab products found in correlation

Mantra quantitative pathology workstation, by PerkinElmer (2 mentions) Cd56 clone 123c3, by Agilent Technologies (1 mentions) Pan cytokeratin clone ae1 ae3, by Agilent Technologies (1 mentions) Cd3 clone f7.2.38, by Agilent Technologies (1 mentions) Inform image analysis software, by PerkinElmer (1 mentions)

Spelling variants of this product

Opal 7-Color Manual IHC Kit (36 citations) Opal 7-Color IHC Kit (6 citations) Opal 7 Kit (6 citations) Opal® 7-color Kit (2 citations)

2 protocols using opal 7 colors manual ihc kit

Multiplex Immunohistochemistry Analysis of MCL3-PDX

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Multiplex fluorescence immunohistochemistry (mIHC) analysis was carried out on 4 µm thick formalin fixed and paraffin embedded (FFPE) tissue sections from MCL3-PDX samples, using the Opal 7-colors manual IHC kit (PerkinElmer). The slides were stained with the combinations of primary antibody-Opal tyramide signal amplification (TSA) reported in online supplemental table 2, and with Spectral DAPI (FB1490, Perkin Elmer, Massachusetts, USA). Stained slides were scanned at 20X using the Mantra Quantitative Pathology Workstation (PerkinElmer), and analyzed with InForm 4.9 Image Analysis Software (V.2.4.2, PerkinElmer). Results are represented as the mean of cell counts/mm² of all analyzed slides for each experimental group. An average of 80 snaps was collected for each experimental group.

Pietà A.D., Cappuzzello E., Palmerini P., Ventura A., Visentin A., Astori G., Chieregato K., Mozzo V., Perbellini O., Tisi M.C., Trentin L., Visco C., Ruggeri M., Sommaggio R, & Rosato A. (2021). Innovative therapeutic strategy for B-cell malignancies that combines obinutuzumab and cytokine-induced killer cells. Journal for Immunotherapy of Cancer, 9(7), e002475.

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Multiplex Immunohistochemistry for Immune Profiling

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mIHC analysis was carried out on 4 µm-thick FFPE tissue sections, using the Opal 7-colors manual IHC kit (PerkinElmer, NEL811001KT). The slides were stained with CD3 (clone F.7.2.38, Dako), CD56 (clone 123 C3, Dako), CD16 (clone 2 H7, Leica Biosystems), pan-cytokeratin (clone AE1/AE3, Dako) antibodies and DAPI. Multiplex stained slides were scanned at 20X using the Mantra Quantitative Pathology Workstation (PerkinElmer), and analyzed with InForm Image Analysis Software (PerkinElmer, v2.4.2).

Sommaggio R., Cappuzzello E., Dalla Pietà A., Tosi A., Palmerini P., Carpanese D., Nicolè L, & Rosato A. (2020). Adoptive cell therapy of triple negative breast cancer with redirected cytokine-induced killer cells. Oncoimmunology, 9(1), 1777046.

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