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Cd64 apc

Manufactured by Thermo Fisher Scientific
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The CD64-APC is a lab equipment product that is used for the detection and identification of CD64-positive cells in biological samples. It functions by utilizing a fluorescent-labeled antibody specific to the CD64 surface antigen. This allows for the accurate quantification and characterization of CD64-expressing cells through flow cytometry analysis.

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Lab products found in correlation

Cd273 pe, by Thermo Fisher Scientific (1 mentions) Cd86 pe cy5, by Thermo Fisher Scientific (1 mentions) Facscalibur, by BD (1 mentions) Cd4 fitc, by Thermo Fisher Scientific (1 mentions) Cd4 apc, by Thermo Fisher Scientific (1 mentions) Cd8 pe, by Thermo Fisher Scientific (1 mentions) Cd163 pe, by Thermo Fisher Scientific (1 mentions) Cd206 fitc, by Thermo Fisher Scientific (1 mentions) Cd40 fitc, by Thermo Fisher Scientific (1 mentions) Hla dr pe cy5, by Thermo Fisher Scientific (1 mentions) Cd274 apc, by Thermo Fisher Scientific (1 mentions) Cd14 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd24 pe, by Beckman Coulter (1 mentions) Cd15 e fluor 450, by Thermo Fisher Scientific (1 mentions) Facsdiva software, by BD (1 mentions) Cd45 percp, by BD (1 mentions) Cd68 pe, by Thermo Fisher Scientific (1 mentions) Cd206 alexa fluor 488, by Thermo Fisher Scientific (1 mentions) F4 80 apc, by Thermo Fisher Scientific (1 mentions) Lsrii flow cytometry, by BD (1 mentions)

3 protocols using cd64 apc

1

Multiparametric Flow Cytometry

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The following directly conjugated anti-human monoclonal antibodies were used: CD4-FITC, CD4-APC, CD8-PE, CD163-PE, CD206-FITC, CD86-PE-Cy5, CD64-APC, CD273-PE, TLR-4-PE-Cy7, CD40-FITC, HLA-DR-PECy5 and CD274-APC (eBioscience, San Diego). Saturating amounts of antibody were used to stain approximately 3 x 105 cells in staining buffer (1 x PBS, 2% FBS) at a final volume of 20 μl for 30 min at 4°C protected from the light. All samples were washed with staining buffer and resuspended in 200 μl of FACS Buffer. Samples were examined in a FACSCalibur (BD Biosciences) and analysis was performed using FlowJo software (Tree Star, Inc., OR).
Ortiz M.C., Lefimil C., Rodas P.I., Vernal R., Lopez M., Acuña-Castillo C., Imarai M, & Escobar A. (2015). Neisseria gonorrhoeae Modulates Immunity by Polarizing Human Macrophages to a M2 Profile. PLoS ONE, 10(6), e0130713.
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2

Immunophenotyping of Cell Populations

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CD59 PE was purchased from Invitrogen, CD235a and CD24PE from Beckman Coulter, CD45PERCP from Becton Dickinson, and CD64APC, CD14PE-Cy7 and CD15 e-fluor 450 from e Bioscience. Fluorescent Aerolysin (FLAER) was obtained from Cedarlane, and lysing reagent (pharmalyse) from Becton Dikinson. For flow studies, acquisition was performed using the FACS Canto II instrument and analysis was performed using the BD FACSDiva™software
Pode-Shakked B., Heimer G., Vilboux T., Marek-Yagel D., Ben-Zeev B., Davids M., Ferreira C.R., Philosoph A.M., Veber A., Pode-Shakked N., Kenet G., Soudack M., Hoffmann C., Vernitsky H., Safaniev M., Lodzki M., Lahad A., Shouval D.S., Levinkopf D., Weiss B., Barg A.A., Daka A., Amariglio N., Malicdan M.C., Gahl W.A, & Anikster Y. (2019). Cerebral and portal vein thrombosis, macrocephaly and atypical absence seizures in Glycosylphosphatidyl Inositol deficiency due to a PIGM promoter mutation. Molecular genetics and metabolism, 128(1-2), 151-161.
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3

Phenotypic Analysis of Mouse and Human Macrophages

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After isolation, the peritoneal- and bone marrow-derived macrophages from mice were culture and analyzed by FACS using the following antibodies: F4/80-APC, CD206-Alexa Fluor 488, CD80-PE, anti-mouse CD86-PE, CD11c-APC (eBioscience). Human macrophages were analyzed following Fc receptor blocking with Human TruStain FcX™ (Biolegend), using antibodies to CD64-APC, CD163-PE-Cyanine7, CD206-Alexa Fluor 488, and CD68-PE (eBioscience). Data were collected by LSR II flow cytometry (BD) and analyzed with FlowJo software (FlowJo Enterprises, Ashland OR).
Tripathi M., Nandana S., Billet S., Cavassani K.A., Mishra R., Chung L.W., Posadas E.M, & Bhowmick N.A. (2017). Modulation of cabozantinib efficacy by the prostate tumor microenvironment. Oncotarget, 8(50), 87891-87902.
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