Accu chek go glucometer

Rats were fasted overnight and then received an intraperitoneal (IP) single dose of streptozotocin (STZ, 60 mg/kg bw) solution dissolved in cold sodium citrate buffer (0.1 M, pH 4.6). After 3 days, the tail vein fasting blood glucose (FBG) level was assessed using an ACCU–CHEK Go glucometer (Roche Co, Mannheim, Germany). Only rats with FBG over 200 mg/dL were considered diabetics [24 (link)].

Blood glucose levels were measured in blood drops from the lateral tail vein using an Accu-Chek Go glucometer (Roche, Basel, Switzerland). Basal blood glucose concentration was assessed after 6 h of fasting (starting at 9 a.m.) before starting and ending treatment with the diets. Acute changes in the glycemic induced by the diets were evaluated after 10 h of fasting at 30, 60, 90, and 120 min after starting refeeding.

Glucose tolerance tests (GTT) were performed on 6 h-fasted mice that were administered i.p. with glucose (1 g/kg body weight). Blood glucose levels were assessed at 0, 15, 30, 60, and 90 min after glucose administration using a Accu-chek Go glucometer (Roche, Dee Why, Australia). Serum during GTT was collected and stored for subsequent insulin assays using insulin RIA kits (Linco Research). Fed and fasted serum insulin levels were also measured using insulin RIA kits (Linco Research). For islet transplantation, mice were fasted overnight and i.v. administered with glucose (1 g/kg body weight) and blood glucose levels were assessed at 0, 2, 5, 10, 15, 30, and 60 min after glucose administration using a glucometer. Blood samples during GTT were collected and stored for subsequent insulin assays using an ultra-sensitive commercially available ELISA kit (Crystal Chem, Downers Grove, USA). Human insulin levels during human islet transplantation were determined using human insulin ELISA kit (Mercodia AB, Sweden).