Pac 1 fitc antibodies

Myricetin, gallic acid, ADP, thrombin, phorbol-12-myristate-13-acetate (PMA), thrombin Receptor Activator Peptide 6 (TRAP-6), human fibrinogen, and 1,4-Dithiothreitol (DTT) and 3,3′-Dihexyloxacarbocyanine iodide (DIOC₆) were purchased from Sigma-aldrich (Dorset, UK). PAPA-NONOate was purchased from Tocris (Abingdon, UK). PE/Cy5 anti human CD62P and PAC-1 FITC antibodies were purchased from BD Biosciences (Wokingham, UK). FITC-conjugated fibrinogen was purchased from Agilent (Stockport, UK). Collagen was purchased from Nycomed (Munich, Germany) whereas Collagen-Related Peptide (CRP) was obtained from Prof Richard Farndale (University of Cambridge, Cambridge, UK). Anti-phospho-vasodilator-stimulated phospho-protein (VASP) (Ser239) was purchased from Cell Signalling (Hitchin, UK), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from Proteintech (Manchester, UK), and Alexa-488 conjugated phalloidin secondary antibody was bought from Life Technologies (Paisley, UK)

Thrombin (Roche, Mannheim, Germany), convulxin (Axxora, Lörrach, Germany), Forskolin, Calyculin A, H89, thymoquinone, ODQ, IBMX, and SQ22536 (Sigma-Aldrich, Munich, Germany), DEA-NO (Alexis Biochemicals, Lörrach, Germany), Rp-8-Br-cAMPS and Rp-8-Br-PET-cGMPS (BioLog, Bremen, Germany), phospho-VASP^S239 (Nano Tools, Teningen, Germany), phospho Ser/Thr and actin antibodies (Cell Signaling, Frankfurt, Germany), horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (Amersham, Freiburg, Germany), Annexin-V-PE, P-selectin, rabbit anti-active caspase-3-PE, CD41-FITC, and PAC-1-FITC antibodies (BD-Bioscience, Heidelberg, Germany), JC1 (Invitrogen, Eugen, Germany), ABT-737 (Selleckchem, Munich, Germany), z-VAD-fmk and z-DEVD-fmk (Calbiochem, Schwalbach, Germany), calcein-AM (Molecular Probes, Gottingen, Germany) were purchased. PKA type II holoenzyme was purified from bovine heart as described.^{64 (link)} C-subunit of PKA antibody was a kind gift of G. Schwoch, Göttingen (Germany). Active caspase-3 (ENZO ALX-201-059) and caspase-3-specific substrate Ac-DEVD-pNA (ENZO ALX-260-033) were from ENZO Life Sciences (Lörrach, Germany).