The Grn-A antibody (Novus, 26,320,002) was used for ICF (1:100), IHF (1:100), IHC (1:500) and WB (1:1000). The polyclonal goat PGRN antibody (R&D systems, AF2420) was used for WB (1:1000). Cat L antibody (R&D Systems, AF952) was used for ICF (1:200), IHF (1:500) and WB (1:2000). Lamp2 antibody (BD Biosciences, clone H4B4) was used for ICF (1:300). Lamp1 antibody (Santa Cruz, clone E-5) was used for IHF (1:100). NeuN (EMD Millipore, clone A60) was used for IHF (1:100). Cathepsin D polyclonal antibody (Athens Research & Technology, 01-12-030104) was used for WB (1:2000). The cathepsin D antibody (Santa cruz, clone C-20) was also used for WB (1:200). The cathepsin B antibody (R&D systems, AF953) was used for WB (1:1000). GAPDH antibody (Meridian, H86504M) was used for WB (1: 10,000).
Lamp1 antibody
Manufactured by Santa Cruz Biotechnology
The LAMP1 antibody is a laboratory reagent used for the detection and analysis of the LAMP1 protein. LAMP1 is a lysosomal membrane protein that plays a role in the trafficking and fusion of lysosomes. The LAMP1 antibody can be used in various research applications, such as Western blot, immunohistochemistry, and immunofluorescence, to study the expression and localization of the LAMP1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710 confocal microscope, by Zeiss (3 mentions)
Neuronal nuclei (neun), by Merck Group (1 mentions)
Lab tek 2, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 goat anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions)
Tamra azide, by Thermo Fisher Scientific (1 mentions)
Eclipse te2000 e, by Nikon (1 mentions)
Control sirna, by RiboBio (1 mentions)
H 1000, by Vector Laboratories (1 mentions)
Eea1 antibody, by BD (1 mentions)
A11008, by Thermo Fisher Scientific (1 mentions)
A11001, by Thermo Fisher Scientific (1 mentions)
4 protocols using lamp1 antibody
1
Antibody Usage Protocols for Western Blot, Immunocytochemistry and Immunohistochemistry
2
LASV Inhibitor Screening and Localization
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Stock solutions of LASV inhibitor 3.3 and 3.3 analogs 3.0, 100 and 1519 were diluted in media to the indicated concentrations (final DMSO 1.1%). Vero cells or Huh7.5 cells seeded on chambered coverglass (Nunc Lab-Tek II) were incubated with the compounds for 1h at 37°C in the dark. Cells were irradiated for 7min at 365nm followed by 3min at 302nm. Cells were then fixed with formalin and click chemistry was performed using 100μM TBTA, 2mM CuSO4, 1mM TCEP and 25μM TAMRA azide (Thermo Fisher) in 50mM Tris pH 8 by incubating at room temperature for 1h in the dark. Following click chemistry, cells were washed with PBS and permeabilized with 0.2% Triton and blocked with 1% BSA. LAMP1 was visualized using a mouse monoclonal LAMP1 antibody (Santa Cruz Biotechnology) followed by a goat anti-mouse Alexa Fluor 488 secondary antibody (Thermo Fisher). Cells were imaged on a Nikon Eclipse TE2000E.
3
Trastuzumab and gp96 Protein Expression
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Trastuzumab was purchased from pharmacy. gp96 antibodies and LAMP-1 antibody were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). The Hsp90α antibody,Hsp90β antibody andα-tubulin antibody were purchased from Bioworld Technology. All other antibodies were purchased from Cell Signaling Technology (Beverly, Massachusetts). The gp96-specific siRNA and control siRNA were designed and synthesized by RiboBio Co., Ltd. (Guangzhou, China). gp96 siRNA was a 25-nucleotide duplexes and had the following sequence: 5’-AAGUUGAUGAACUGAGAGUACUUCC-3’.
4
Quantifying Protein Colocalization in Cells
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GFP-LC3B cells were grown on sterilized glass coverslips. After drug treatment, cells were fixed with 4% paraformaldehyde. For immunostaining, cells were blocked with 10% goat serum (Gibco, 16210) in phosphate-buffered saline (PBS; Wellgene, ML008), stained with a 1:500 dilution of primary antibody in PBS, and stained with a 1:1000 dilution of fluorescence-conjugated secondary antibody (Invitrogen, A11001, A11008, A11011, A11004, A11045, A11046). Finally, slides were washed 3 times with PBS, stained with DAPI and mounted in mounting medium (Vector, H-1000). Images were captured with a Carl Zeiss LSM710 confocal microscope (Oberkochem, Germany). The EEA1 antibody was purchased from BD (610457), and the LAMP1 antibody from Santa Cruz Biotechnology (sc-20011). To measure the extent of protein colocalization, confocal images were quantified using the Pearson correlation coefficient (PCC) as described previously.32,33 (link) PCC (Rr) values were calculated by WCIF ImageJ software (NIH, Bethesda, MD). The correlation coefficient was calculated from 5 cells per group.
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