Cfi plan apo lambda 60x oil

Manufactured by Nikon

The CFI Plan Apo Lambda 60X Oil is a high-performance microscope objective lens designed for use in various laboratory applications. It provides a 60X magnification and is optimized for use with oil immersion. The lens features a numerical aperture of 1.40, enabling high-resolution imaging. Its plan-apochromatic design ensures minimal distortion and chromatic aberration, delivering sharp, high-contrast images.

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Lab products found in correlation

Alexa flour 647, by Thermo Fisher Scientific (2 mentions) C2 confocal scan head, by Nikon (1 mentions) Nis elements 5, by Nikon (1 mentions) Eclipse ti e microscope, by Nikon (1 mentions) Alexa flour 568, by Thermo Fisher Scientific (1 mentions) Fluoroshield, by Merck Group (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CFI Plan Apo Lambda Plan Apo 60x Plan Apo Lambda Plan Apo

3 protocols using cfi plan apo lambda 60x oil

Confocal Imaging of DNA Double-Strand Breaks

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Confocal images were captured with a Nikon C2+ confocal scan head attached to a Nikon Eclipse Ti-E microscope. Confocal images were captured using a high NA, PLAN 60X objective (Nikon CFI Plan Apo Lambda 60X Oil with NA = 1.4), which enabled us to create high resolution images with a larger number of cell nuclei in the FOV throughout the experiments than during dSTORM measurements. The setup and data acquisition process were controlled by the Nikon NIS-Elements 5.02 software and the captured images were postprocessed in MATLAB. The Nikon Laser Unit was used to set the wavelengths and the power of the applied lasers operated at 405 nm (P_max = 60 mW; Nichia) and 647 nm (2RU-VFL-P-300-647-B1, P_max = 300 mW, MPB Communications Ltd).
All DSBs induced in the cell nuclei (N = 49–125) were displayed and counted in 3D.

Brunner S., Varga D., Bozó R., Polanek R., Tőkés T., Szabó E.R., Molnár R., Gémes N., Szebeni G.J., Puskás L.G., Erdélyi M, & Hideghéty K. (2021). Analysis of Ionizing Radiation Induced DNA Damage by Superresolution dSTORM Microscopy. Pathology and Oncology Research, 27, 1609971.

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Immunostaining of Drosophila Larvae

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Third instar larvae were dissected, fixed and stained as previously described (47 (link)). Briefly, larvae or adult were dissected in iced-cold phosphate buffered saline (PBS) (Lonza, #17–516F). Dissected larvae were fixed in 4% formaldehyde, washed 3× in PBS, then washed 3× in 0.1% PBST (0.1% Triton X-100 in PBS) and incubated overnight with primary antibodies (mouse anti-lamin, DSHB, 1:200, mouse anti-mCherry 1:100 rabbit anti-TDP-43, Proteintech 10782-2-AP, 1:200; mouse anti-mono and poly ubiquitinylated conjugates, Enzo Life Sciences BML-PW8810, 1:100; rat anti-phospho Ser409/410 TDP-43, EMD Millipore clone 1D3, 1:50) in 0.1% PBST. Then, Larvae were washed 3× in 0.1% PBST (0.1% Triton X-100 in PBS) and incubated for 2 hours in secondary antibodies (anti-rabbit Alexa Flour 568, Invitrogen, # 651727, 1:100; anti-mouse Alexa Flour 647, Invitrogen, # 28181, 1:100; anti-rabbit Alexa Flour 405, Life Technologies, # 157554, 1:100; anti-rabbit Alexa Flour 647, Life Technologies, # 1660844, 1:100; anti-rat Alexa Fluor 488, Invitrogen, #A-11006), and mounted using Fluoroshield (SIGMA, #F6182). Slides were visualized using a Nikon A1 laser-scanning confocal microscope system utilizing 60X oil immersion objectives (CFI Plan Apo Lambda 60X Oil, Nikon).

Otte C.G., Fortuna T.R., Mann J.R., Gleixner A.M., Ramesh N., Pyles N.J., Pandey U.B, & Donnelly C.J. (2020). Optogenetic TDP-43 nucleation induces persistent insoluble species and progressive motor dysfunction in vivo. Neurobiology of disease, 146, 105078.

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Confocal and Super-Resolution Microscopy

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Image acquisition of fixed samples were acquired on a Nikon A1 laser-scanning confocal system with 40X and/or 60X oil immersion objectives (CFI Plan Fluor 40X Oil; CFI Plan Apo Lambda 60X Oil, Nikon) or Nikon N-SIM super-resolution Microscope with 60X oil immersion objectives (Plan Apo TIRF 60X Oil) and Hamamatsu C11440 Orca Flash 4.0 camera. 3D SIM images were reconstructed and then deconvolved prior to analysis. Image analysis was conducted in NIS-Elements AR Analysis 4.51.

Gleixner A.M., Verdone B.M., Otte C.G., Anderson E.N., Ramesh N., Shapiro O.R., Gale J.R., Mauna J.C., Mann J.R., Copley K.E., Daley E.L., Ortega J.A., Cicardi M.E., Kiskinis E., Kofler J., Pandey U.B., Trotti D, & Donnelly C.J. (2022). NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility. Nature Communications, 13, 3380.

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