L929 cell conditioned media

Manufactured by Corning

L929-cell conditioned media is a laboratory product derived from the culture of L929 cells. It provides a source of various growth factors and other biomolecules that may be useful in cell culture applications. The core function of this product is to supplement cell culture media, but its specific intended uses should not be extrapolated.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Corning (2 mentions) L glutamine, by Corning (2 mentions) Hela cell, by American Type Culture Collection (1 mentions) Vero cell, by American Type Culture Collection (1 mentions) J774 cells, by American Type Culture Collection (1 mentions) Antibiotics penicillin streptomycin, by Corning (1 mentions) Penicillin streptomycin, by Corning (1 mentions) B6.129s4 mbl1tm1kata mbl2tm1kata j, by Jackson ImmunoResearch (1 mentions)

2 protocols using l929 cell conditioned media

Generation of Bone Marrow-derived Macrophages

Cited 1 time

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Hela cells, Vero cells and J774 cells were purchased from ATCC. Bone marrow-derived macrophages (BMDMs) were generated from hematopoietic stem cells isolated from CD45.1⁺ or CD45.2⁺ mice bone marrow, and maintained in DMEM (Corning Cellgro) containing 10% FBS (Thermo Scientific), 10% L929-cell conditioned media (containing M-CSF), 2 mM L-glutamine, 1 mM sodium pyruvate and antibiotics (penicillin/streptomycin) (Corning cellgro), at 37C in a 5% CO2 incubator. After 6-day culture, cells were either split for further expansion or frozen with FBS containing 10% DMSO in liquid nitrogen for later use (38 (link)).

Xu Y., Huang L., Kirschman J.L., Vanover D.A., Tiwari P.M., Santangelo P.J., Shen X, & Russell D.G. (2018). Exploitation of synthetic mRNA to drive immune effector cell recruitment and functional re-programming in vivo.. Journal of immunology (Baltimore, Md. : 1950), 202(2), 608-617.

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Bone Marrow-Derived Macrophage Isolation

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C57BL/6J mice, MBL knockout mice (B6.129S4-Mbl1^tm1Kata Mbl2^tm1Kata/J) were obtained from the Jackson Laboratory and housed under pathogen-free conditions.
Bone marrow-derived macrophages (BMDMs) were isolated from bone marrow of C57BL/6J wild type mice, and maintained in DMEM (Corning Cellgro) containing 10% FBS (Thermo Scientific), 10% L929-cell conditioned media, 2mM L-glutamine, 1mM sodium pyruvate and antibiotics (penicillin/streptomycin) (Corning cellgro), at 37^°C in a 5% CO2 incubator[64 (link), 65 (link)].

Xu Y., Wang L., Zimmerman M.D., Chen K.Y., Huang L., Fu D.J., Kaya F., Rakhilin N., Nazarova E.V., Bu P., Dartois V., Russell D.G, & Shen X. (2018). Matrix metalloproteinase inhibitors enhance the efficacy of frontline drugs against Mycobacterium tuberculosis. PLoS Pathogens, 14(4), e1006974.

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