Rabbit polyclonal anti beta actin

Pegylated human recombinant Arginase I cobalt [HuArgI (Co)-PEG5000] (Pegzilarginase) was a gift from Aeaglea BioTherapeutics (Aeaglea BioTherapeutics, Austin, TX, USA). The following primary antibodies were used in this study: Rabbit polyclonal anti-beta Actin, mouse monoclonal anti-MMP2[6E3F8], rabbit polyclonal anti-MMP9, rabbit polyclonal anti-ASS-1, and mouse monoclonal anti-vinculin [VIN-54] (Abcam Inc., Cambridge, UK). The following secondary antibodies were used in this study: Alexa Fluor 488 goat anti-mouse IgG (H + L) was obtained from Invitrogen). Anti-rabbit and anti-mouse HRP-conjugated secondary antibodies were obtained from Promega (Promega Co., Madison, WI, USA).

8–10% polyacrylamide gels were used for protein separation and detection. The gels were transferred to a nitrocellulose membrane, and antibody hybridization and electrochemiluminescence (ECL) were performed according to standard procedures. The primary antibodies used in this analysis were rabbit monoclonal anti-phosphorylated ATM 1:1,000 (Abcam), mouse monoclonal anti-ATM 1:10,000 (sigma), rabbit monoclonal anti-phosphorylated CHK1 1:200 (Cell Signaling), mouse monoclonal anti-CHK1 1:500 (Abcam), mouse monoclonal anti-cyclin E 1:200 (Santa Cruz Biotechnology), and rabbit polyclonal anti-beta actin 1:5,000 (Abcam). HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were obtained from Jackson ImmunoResearch laboratories (West Grove, PA).