Kidneys were harvested from mice at the end of the study (at 43–44 weeks of age). For light microscopy, the tissues were fixed in 10% neutral buffered formalin, embedded in paraffin wax, sectioned at a thickness of 5 μm and stained with H&E (BBC Biochemical, Mount Vernon, WA, USA). Periodic acid/Schiff (PAS stain kit, BBC Biochemical) staining and Masson trichrome (BBC Biochemical) staining were used for the detection of proteinuria and fibrosis, respectively. Inflammatory cell infiltration and mesangial proliferation were scored on a 4-point scale as 0 (none), 1+ (mild), 2+ (moderate), 3+ (moderate to severe) and 4+ (severe).
Periodic acid schiff pas stain kit
Manufactured by StatLab
The Periodic acid/Schiff (PAS) stain kit is a laboratory tool used for the detection of polysaccharides, glycoproteins, and other carbohydrate-containing substances in tissue samples. The kit provides the necessary reagents and protocols to perform this staining technique.
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2 protocols using periodic acid schiff pas stain kit
1
Kidney Histopathology in Aged Mice
2
Histological Analysis of Aged Murine Kidneys
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Kidneys were harvested from mice that died of natural causes immediately upon death or at the end of the study (at 62 weeks of age). For light microscopy, the tissues were fixed in 10% neutral buffered formalin, embedded in paraffin wax, sectioned at a thickness of 5 μm, and stained with H&E (BBC Biochemical, Mount Vernon, WA, USA) by using routine histological techniques. Periodic acid/Schiff (PAS stain kit; BBC Biochemical) staining and Masson trichrome (BBC Biochemical) staining were used for the detection of proteinuria and fibrosis, respectively.
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