Htc esquire spectrometer

Optical rotations were measured with a JASCO P-1020 digital polarimeter. UV
spectra were obtained using a Shimadzu UV-2401 PC spectrophotometer. IR spectra
were recorded on a Bruker Tensor-27 infrared spectrophotometer using KBr pellets.
1D and 2D NMR spectra were performed on Bruker AM-400 and DRX-500 spectrometers
(Bruker BioSpin GmBH, Rheinstetten, Germany) with TMS as the internal standard.
ESIMS spectra were recorded on a Bruker HTC/Esquire spectrometer. HREIMS was
recorded on a Waters AutoSpec Premier P776 spectrometer. Column Chromatography
(CC) was performed on silica gel (200–300 mesh, Qingdao Marine Chemical Ltd.,
Qingdao, China), Rp-18 (40–63 μm, Merk). Fractions were monitored by TLC (GF254,
Qingdao Marine Chemical Ltd., Qingdao, China), and by heating silica gel plates
sprayed with 10% H₂SO₄ in ethanol.
GC analysis was performed on an HP5890 gas chromatograph equipped with a
H₂ flame ionization detector.

Optical rotations were measured with a JASCO P-1020 digital polarimeter. UV spectra were obtained using a Shimadzu UV-2401 PC spectrophotometer. IR spectra were recorded on a Bruker Tensor-27 infrared spectrophotometer using KBr pellets. 1 day and 2D NMR spectra were performed on Bruker AM-400 and DRX-500 spectrometers (BrukerBioSpinGmBH, Rheinstetten, Germany) with TMS as the internal standard. ESIMS spectra were recorded on a Bruker HTC/Esquire spectrometer. HREIMS was recorded on a Waters AutoSpec Premier P776 spectrometer. Column Chromatography (CC) was performed on silica gel (200–300 mesh, Qingdao Marine Chemical Ltd., Qingdao, China), Rp-18 (40–63 μm, Merk). Fractions were monitored by TLC (GF254, Qingdao Marine Chemical Ltd., Qingdao, China), and by heating silica gel plates sprayed with 10% H₂SO₄ in ethanol. GC analysis was performed on an HP5890 gas chromatograph equipped with a H₂ flame ionization detector.