Cd24 pe clone m1 69

Manufactured by Thermo Fisher Scientific

CD24-PE (clone M1/69) is a fluorescently labeled antibody used for the detection and analysis of CD24 (heat-stable antigen) expression on cells. It is a useful tool for flow cytometry applications.

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Lab products found in correlation

Cd44 fitc, by Thermo Fisher Scientific (1 mentions) Cd73 biotin clone ty 11, by BioLegend (1 mentions) Facsaria 3 sorter, by BD (1 mentions) Clone g8, by BioLegend (1 mentions) Epcam pe cy7, by BioLegend (1 mentions) Streptavidin apc cy7, by BioLegend (1 mentions) Cd45 clone 30 f11, by Thermo Fisher Scientific (1 mentions) Cd34 clone ram34, by Thermo Fisher Scientific (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions)

Close spelling variants of this product

CD24 (M1/69) Clone M1/69 M1/69 CD24-PE

2 protocols using cd24 pe clone m1 69

Mesenchymal Stem Cell Phenotyping

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Antibodies used for phenotyping include: CD24-PE (clone M1/69 eBioscience Inc. San Diego CA), CD34-FITC (clone 1H6 Serotech, Raleigh, NC), CD44-FITC (clone 1M7 eBioscience Inc. San Diego CA), CD45-FITC (clone YKIX716.13 Serotech, Raleigh, NC), CD73-biotin (clone TY/11.8, Biolegend. San Diego CA), CD90-APC (clone YKIX337.217, eBioscience Inc., San Diego CA), Oct3/4 (clone EM92, eBioscience Inc. San Diego CA), and unconjugated CD105 (clone 8A1, Abcam. Cambridge, MA).

Chow L., Johnson V., Regan D., Wheat W., Webb S., Koch P, & Dow S. (2017). Safety and immune regulatory properties of canine induced pluripotent stem cell-derived mesenchymal stem cells. Stem cell research, 25, 221-232.

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Mouse lung single-cell flow cytometry

Cited 1 time

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Mouse lung single-cell suspensions and the procedures of staining the cells for flow cytometry and data analysis were described previously (Barkauskas et al., 2013 (link); Chen et al., 2012 (link); Liang et al., 2016 (link)). Flow cytometry was performed using a LSRFortessa flow cytometer and FACSAria III sorter (BD, San Jose, CA) and the data were analyzed using Flow Jo 9.9.6 software (Tree Star, Ashland, OR). Primary antibodies EpCAM-PE-Cy7 (clone G8.8, Catalog # 118216, RRID AB_1236471) were from BioLegend. CD24-PE (clone M1/69, Catalog # 12-0242-82, RRID AB_467169), Sca-1 (Ly-6A/E)-APC (clone D7, Catalog # 17-5981-82, RRID AB_469487), CD31 (PECAM-1) (clone 390, Catalog # 13-0311-85, RRID AB_466421), CD34 (clone RAM34, Catalog # 13-0341-85, RRID AB_466425), and CD45 (clone 30-F11, Catalog # 13-0451-85, RRID AB_466447) were all from eBioscience (San Diego, CA). Streptavidin-APC-Cy7 (catalog # 405208) was from BioLegend (San Diego, CA).

Liang J., Huang G., Liu X., Liu N., Taghavifar F., Dai K., Yao C., Deng N., Wang Y., Chen P., Hogaboam C., Stripp B.R., Parks W.C., Noble P.W, & Jiang D. (2023). Reciprocal interactions between alveolar progenitor dysfunction and aging promote lung fibrosis. eLife, 12, e85415.

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