70 μm and 40 μm cell strainers

Manufactured by BD

Sourced in United States

The 70-μm and 40-μm cell strainers are laboratory equipment used for separating and isolating cells from a cell suspension. They function by mechanically filtering the suspension through a mesh screen with the specified pore size, allowing the smaller cells and cellular components to pass through while retaining the larger cells.

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Lab products found in correlation

Cd45 apc cy7, by BD (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Facsdiva, by BD (1 mentions) Aria 1, by BD (1 mentions) Chelexed fcs, by Merck Group (1 mentions) Collagenase type 1, by Worthington (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Merck Group (1 mentions) Penicillin, by Merck Group (1 mentions)

Close spelling variants of this product

70-μm cell strainer (905 citations) Cell strainer (717 citations) 40-μm cell strainer (575 citations) 70-μm strainer (108 citations) 40-μm strainer (62 citations) μm cell strainers (7 citations) 70 and 40 μm cell strainers (6 citations)

2 protocols using 70 μm and 40 μm cell strainers

Epidermal Cell Isolation and Sorting

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Epidermal cell suspensions were generated by surgically removing the trunk skin and gently scraping off subcutaneous adipose tissue using a scalpel knife. Skin was then placed dermal side down on DMEM (Gibco) containing 2 mM EDTA and 0.25% Trypsin (Gibco) at 4 °C overnight. The next day, the epidermis was softly scraped off and further dissociated into single cells by pipetting up and down after adding DMEM containing 2% chelexed FCS (Sigma). Cells were filtered using 70-μm and 40-μm cell strainers (BD), centrifuged at 300 × g for 10 min and washed with PBS (Sigma) containing 2% chelexed FCS twice before staining. Epidermal single-cell suspensions were incubated with the following antibodies on ice for 30 min: a6-PE (BD, 1:50), Scal-PECy7 (Biolegend, 1:100) and CD45-APCCy7 (BD, 1:200). Dead cell exclusion was performed using Hoechst (1 μg/ml). Cell sorting of ~8,000 keratinocytes derived from interfollicular epidermis (IFE) (characterised as a6 high, ScaI high) from individual P2 pups was performed using a BD AriaI and BD FACSDiva software.

Hippe A., Braun S.A., Oláh P., Gerber P.A., Schorr A., Seeliger S., Holtz S., Jannasch K., Pivarcsi A., Buhren B., Schrumpf H., Kislat A., Bünemann E., Steinhoff M., Fischer J., Lira S.A., Boukamp P., Hevezi P., Stoecklein N.H., Hoffmann T., Alves F., Sleeman J., Bauer T., Klufa J., Amberg N., Sibilia M., Zlotnik A., Müller-Homey A, & Homey B. (2020). EGFR/Ras-induced CCL20 production modulates the tumour microenvironment. British Journal of Cancer, 123(6), 942-954.

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Isolation of Adipose-Derived Mesenchymal Stem Cells

Cited 1 time

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Adipose-derived mesenchymal stem cells (ASCs) were isolated from human adipose tissues obtained after informed donor consent and with approval from the Mayo Clinic Institutional Review Board (IRB), as previously described.^{48 (link),49 (link)} Briefly, adipose tissue was digested with 0.075% collagenase type I (Worthington Biochemicals, Lakewood, NJ, USA) for 90 minutes at 37°C, and adipocytes removed from the stromal vascular fraction by low-speed centrifugation at 400 g for 5 minutes. The resulting cell pellet was washed with PBS followed by sieving through 70-μm and 40-μm cell strainers (BD Biosciences, San Jose, CA, USA). The cell suspension was seeded at densities of 1.0–2.5 × 10³ cells/cm² in T-175 flasks using maintenance media (Advanced MEM) with 5% platelet lysate, PLTMax (MillCreek LifeSciences, USA), 2 U/mL heparin (Novaplus, USA), 2 mmol/L L-glutamine (Invitrogen, Waltham, MA, USA), and antibiotics (100 U/mL penicillin, 100 g/mL streptomycin, Sigma, St Louis, MO, USA) and incubated in standard culture conditions of 37°C and 5% CO₂.

Maria S., Samsonraj R.M., Munmun F., Glas J., Silvestros M., Kotlarczyk M.P., Rylands R., Dudakovic A., van Wijnen A.J., Enderby L.T., Lassila H., Dodda B., Davis V.L., Balk J., Burow M., Bunnell B.A, & Witt-Enderby P.A. (2018). Biological effects of melatonin on osteoblast/osteoclast cocultures, bone, and quality of life: Implications of a role for MT2 melatonin receptors, MEK1/2, and MEK5 in melatonin-mediated osteoblastogenesis. Journal of pineal research, 64(3), 10.1111/jpi.12465.

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