L 1 peptone

Manufactured by Merck Group

Sourced in Sao Tome and Principe, Germany

L-1 peptone is a microbial culture medium ingredient manufactured by Merck Group. It serves as a source of organic nitrogen, vitamins, and other growth factors to support the cultivation of a variety of microorganisms.

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Lab products found in correlation

L 1 agar, by Merck Group (1 mentions) 1 diphenyl 2 picryl hydrazyl dpph, by Merck Group (1 mentions) Nutrient broth, by Merck Group (1 mentions) L 1 nacl, by Merck Group (1 mentions) Yeast extract, by Thermo Fisher Scientific (1 mentions) Dextrose, by Thermo Fisher Scientific (1 mentions) Peptone, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)

3 protocols using l 1 peptone

Microbiological and Antioxidant Evaluation of Polyamide Fabrics

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Nutrient broth (NB) consisting of 3 g L^-1 meat extract, 5 g L^-1 peptone and salts was used as a medium for bacterial cultures. Nutrient agar petri-dishes were prepared with composition 3 g L^-1 meat extract, 5 g L^-1 peptone, and 15 g L^-1 agar. Sabouraud medium was used for maintenance of Candida spp. and it contained 40 g L^-1 glucose, 10 g L^-1 peptone, 20 g L^-1 agar, pH 5.6.
1,1-Diphenyl-2-picryl-hydrazyl (DPPH) was purchased from Sigma (St. Louis, MO, United States). Methanol used was of HPLC grade. Polyamide knitted fabric with density 23 columns cm^-1 and 15 rows cm^-1, weight 12 g m^-2, 154.7 Denier and average thickness 0.309 mm was supplied by Colora S.A. (Thessaloniki, Greece). The PA fabric was washed with detergent Felosan NFG before use for the removal of impurities.

Kanelli M., Mandic M., Kalakona M., Vasilakos S., Kekos D., Nikodinovic-Runic J, & Topakas E. (2018). Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties. Frontiers in Microbiology, 9, 1495.

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Pseudomonas taetrolens LMG 2336 Cultivation

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Pseudomonas taetrolens LMG 2336 (from the Belgian Coordinated Collection of Microorganisms, Ghent, Belgium) was used. The microorganism was inoculated on Nutrient Broth (NB, containing 1 g L⁻¹ meat extract, 2 g L⁻¹ yeast extract, 5 g L⁻¹ peptone and 5 g L⁻¹ NaCl, all from Sigma-Aldrich, Steinheim, Germany) agar (20 g L⁻¹, VWR Chemicals, West Chester, PA, USA) plates and incubated at 30 °C for 48 h. A loopful from a fresh NB agar plate was inoculated in a 500 mL Erlenmeyer flask containing 100 mL of NB broth (Sigma-Aldrich) (ratio medium air 1:4). The inoculum was incubated in an orbital shaker (Model G25; New Brunswick Scientific Co., Edison, NJ, USA) at 250 rpm and 30 °C for 10 h. After this time, biomass was separated by centrifugation (10,000 rpm, 10 min) and was subsequently used as a bulk starter.

Sáez-Orviz S., Marcet I., Rendueles M, & Díaz M. (2022). Preparation of Edible Films with Lactobacillus plantarum and Lactobionic Acid Produced by Sweet Whey Fermentation. Membranes, 12(2), 115.

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Yeast Growth and Harvesting Protocol

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Bakers' yeast (Saccharomyces cerevisiae, Bruggeman) was grown on 15 g L -1 agar (Fisher Scientific) YPD (yeast extract-peptone-dextrose) plates containing 10 g L -1 yeast extract (Fisher Scientific), 20 g L -1 peptone (Sigma-Aldrich) and 20 g L -1 dextrose (Fisher Scientific), or in YPD broth. Agar plates were inoculated from 20% w/v glycerol stock, grown overnight at 30 °C and stored at 4 °C until further use. Liquid cultures were inoculated from single colonies and incubated in shake flasks at 30 °C and 150 rpm. Cells were harvested during the exponential growth phase, when the yeast was budding, by centrifuging at 3220 × g for 10 minutes, washing twice in 1 : 100 phosphate-buffered saline (PBS, Invitrogen, pH 7.4) diluted in Milli-Q water and finally resuspending in the 1 : 100 PBS solution. In the experiments, a concentration of 1-2 × 10 6 cells per ml was used. All EIS measurements were performed on the same day the cells were harvested. Non-budding yeast cells were prepared in the same way as the budding yeast, except they were harvested during the stationary phase. The experiments in this paper were performed on budding yeast cells, unless otherwise stated.

Van den Eeckhoudt R., Christiaens A.S., Ceyssens F., Vangalis V., Verstrepen K.J., Boon N., Tavernier F., Kraft M, & Taurino I. (2023). Full-electric microfluidic platform to capture, analyze and selectively release single cells. Lab on a chip, 23(19).

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