Animal experiments comply with all relevant ethical regulations. All animal experiments were carried out according to the protocol approved by the Institutional Animal Care and Use Committee (IACUC) of Weill Cornell Medical College (protocol number: 2014-0061). ICR female mice were purchased from Taconic Farms (Germantown, NY). Females were superovulated at 6-8 weeks with 10 IU PMSG (pregnant mare serum gonadotrophin, Sigma-Aldrich) and 10 IU hCG (human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 h. The females were mated individually to males and checked for the presence of a vaginal plug the following morning. Plugged females were sacrificed at 1.5 days after hCG injection for collection of 2-cell embryos. These embryos were flushed from the oviducts with KSOM+AA (Specialty Media) and cultured in KSOM for 2.5 days in vitro at 37 °C under 5% CO2 in air to the blastocyst stage.
Human chorionic gonadotrophin
Manufactured by Merck Group
Sourced in Sao Tome and Principe
Human chorionic gonadotrophin (hCG) is a hormone produced during pregnancy. It is used as a laboratory test to detect and monitor pregnancy. hCG is a protein-based hormone that is secreted by the placenta, which is the organ formed in the uterus during pregnancy to nourish the egg after it has been fertilized and becomes attached to the uterine wall.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using human chorionic gonadotrophin
1
Superovulation and Embryo Collection
2
Superovulation and Embryo Collection
3
Ovarian Tissue Grafting for Survival
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To promote graft survival and growth, mice in this phase were subjected to ovariectomy. Fresh, slowfrozen, or vitrified tissues of approximately 4 × 6 × 1 mm 3 were either engrafted into the subcutaneous site on the neck of ovariectomised nude mice, or used for intraperitoneal engraftment in the left abdomen of those mice. 29 Mice in this phase were divided into a saline group and a treatment group after 2 or 6 weeks of engraftment. The presence of gonadotrophins can optimise graft establishment and stimulate follicle growth. 30 To promote folliculogenesis, mice in the treatment group underwent intraperitoneal injection (in the right abdomen) of 1 IU (100 µL) of follitropin alfa (GONAL-f; Merck Serono, Geneva, Switzerland) every other day for 5 to 8 weeks after 2 or 6 weeks' engraftment. 30 During the same period, mice in the saline group underwent intraperitoneal injection (in the right abdomen) of an equal volume of physiological saline every other day. Thirty-six hours before the mice were sacrificed, both groups of mice received a single dose of 10 international units of human chorionic gonadotrophin (Sigma-Aldrich, St Louis [MO], US) by injection to promote ovulation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!