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Sybr green rox pcr master mix

Manufactured by Thermo Fisher Scientific
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Sourced in Germany

SYBR Green/ROX PCR master mix is a ready-to-use solution for real-time PCR amplification. It contains SYBR Green I dye for detecting double-stranded DNA and ROX passive reference dye for signal normalization.

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Lab products found in correlation

Revertaid cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) 7500 real time pcr cycler, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Quantstudio three cycler, by Thermo Fisher Scientific (1 mentions) Rneasy micro kit, by Qiagen (1 mentions)

2 protocols using sybr green rox pcr master mix

1

Quantitative PCR Protocol for MSCs and MNCs

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Total RNA was isolated from MSCs after five days of culture before seeding them on POMA slides for ECM generation as well as from 24h stimulated MNCs using RNeasy Mini Kit (Qiagen, Germany) and reverse transcribed into cDNA using RevertAid cDNA synthesis kit (Thermo-Fisher, Germany) with oligo-dT primers. Relative target quantity was determined using the comparative CT(ΔΔCT) method. RT-PCR was performed using SYBR Green/ROX PCR master mix (Thermo-Fisher, Germany) and target specific primers (Supplementary Table S2) on a 7500 Real-time PCR cycler (Applied Biosystems, Germany). Amplicons were normalized to U6, respectively. All samples were run in duplicates.
Bains A.K., Behrens Wu L., Rivière J., Rother S., Magno V., Friedrichs J., Werner C., Bornhäuser M., Götze K.S., Cross M., Platzbecker U, & Wobus M. (2022). Bone marrow mesenchymal stromal cell-derived extracellular matrix displays altered glycosaminoglycan structure and impaired functionality in Myelodysplastic Syndromes. Frontiers in Oncology, 12, 961473.
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2

Quantifying Gene Expression in CD34+ HSPCs

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Total RNA were isolated from CD34+ HSPCs after incubation with EVs using RNeasy Micro Kit (Qiagen, Germany) and reverse transcribed into cDNA using RevertAid cDNA synthesis kit (Thermo Fisher Scientific, Germany) with oligo-dT primers. Relative target quantity was determined using the comparative CT (∆∆CT) method. RT-PCR was performed using SYBR Green/ROX PCR master mix (Thermo Fisher Scientific, Germany) and target specific primers for PTEN, CDKN2A and SIRT1 (Supplementary Table S4) on a Quantstudio three cycler (Applied Biosystems, Germany). Amplicons were normalized to endogenous GAPDH control. All samples were run in duplicates.
Fichtel P., von Bonin M., Kuhnert R., Möbus K., Bornhäuser M, & Wobus M. (2022). Mesenchymal Stromal Cell-Derived Extracellular Vesicles Modulate Hematopoietic Stem and Progenitor Cell Viability and the Expression of Cell Cycle Regulators in an Age-dependent Manner. Frontiers in Bioengineering and Biotechnology, 10, 892661.
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