All chemicals were from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise specified. The Escherichia coli Rosetta(DE3) strain was purchased from Novagen (Madison, WI, USA). Restriction endonucleases and other cloning reagents were purchased from Fermentas (Glen Burnie, MD, USA). Chelating Sepharose Fast Flow was from Amersham Biosciences (Arlington Heights, IL, USA), and the Isolate II PCR and Gel Kit (Bioline, London, UK). The dye reagent for protein assay was from Bio-Rad (Hemel Hempstead, Herts, UK).
Dye reagent for protein assay
Manufactured by Bio-Rad
Sourced in United Kingdom, United States
The Dye reagent for protein assay is a solution designed for the quantitative determination of protein concentration in biological samples. The reagent contains a dye that binds to proteins, resulting in a color change that can be measured using a spectrophotometer. The intensity of the color is proportional to the protein concentration in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Restriction endonuclease, by Thermo Fisher Scientific (1 mentions)
Isolate 2 pcr and gel kit, by Meridian Bioscience (1 mentions)
Chelating sepharose fast flow, by Cytiva (1 mentions)
Acrylamide bisacrylamide, by Bio-Rad (1 mentions)
N n n n tetramethylethylenediamine temed, by Bio-Rad (1 mentions)
Sodium dodecyl sulphate (sds), by Bio-Rad (1 mentions)
2 mercaptoethanol, by Bio-Rad (1 mentions)
Coomassie brilliant blue r 250, by Bio-Rad (1 mentions)
Bromophenol blue, by Bio-Rad (1 mentions)
Glycerol, by Bio-Rad (1 mentions)
Ammonium persulphate, by Bio-Rad (1 mentions)
2 protocols using dye reagent for protein assay
1
Protein Expression and Purification Protocol
2
SDS-PAGE Reagent Preparation
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Chemicals and solvents were obtained from Fluka Chemie GmbH (Deisenhofen, Germany) and Merck (Darmstadt, Germany). Electrophoresis materials such as bromophenol blue, 2-mercaptoethanol, glycerol, sodium dodecylsulphate (SDS), Coomassie Brilliant Blue R-250, 30% acrylamide/bisacrylamide, ammonium persulphate and N,N,N,N-tetramethylethylenediamine (TEMED) were from Bio-Rad (Hercules, CA, USA). Dye reagent for protein assay (Bio-Rad) and all other reagents were from Sigma (St Louis, MO, USA).
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