Irdye 680 800cw anti

Manufactured by LI COR

The LI-COR IRDye® 680/800CW anti is a near-infrared fluorescent dye that can be conjugated to antibodies or other biomolecules. It has peak excitation and emission wavelengths of 680 and 800 nanometers, respectively.

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Lab products found in correlation

Phosphatase inhibitor, by Cell Signaling Technology (2 mentions) Iblot 2 transfer device, by Thermo Fisher Scientific (2 mentions) Pierce rapid gold bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Laemmli buffer, by Bio-Rad (1 mentions)

Spelling variants of this product

IRDye 800CW (808 citations) IRDye 680 (264 citations) IRDye 680/800cw (2 citations)

2 protocols using irdye 680 800cw anti

Western Blot Protein Quantification

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Cells were lysed in RIPA buffer supplemented with a protease inhibitor cocktail (Roche) and phosphatase inhibitor (Cell Signaling 5870s). Protein were measured with Pierce^TM Rapid Gold BCA Protein Assay Kit (Thermo Fisher A53227) and denatured in 4× Laemmli buffer (Biorad 1610747). Whole cell extracts were resolved by 8–16% Tris-glycine polyacrylamide gel (Invitrogen XP08165BOX), transferred to PVDF membranes with iBlot^TM 2 transfer device (Thermo Fisher IB21001) using P0 protocol, and probed with indicated primary antibodies. Bound antibodies were detected with LI-COR IRDye ® 680/800CW anti-rabbit/mouse secondary antibodies. Primary antibodies listed in the Supplementary Data 5.

Spisak S., Chen D., Likasitwatanakul P., Doan P., Li Z., Bala P., Vizkeleti L., Tisza V., De Silva P., Giannakis M., Wolpin B., Qi J, & Sethi N.S. (2024). Identifying regulators of aberrant stem cell and differentiation activity in colorectal cancer using a dual endogenous reporter system. Nature Communications, 15, 2230.

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Western Blot Protocol for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in RIPA buffer supplemented with a protease inhibitor cocktail (Roche) and phosphatase inhibitor (Cell Signaling 5870s). Protein were measured with Pierce^™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher A53227) and denatured in 4x Laemmli buffer (Biorad 1610747). Whole cell extracts were resolved by 8–16% Tris-glycine polyacrylamide gel (Invitrogen XP08165BOX), transferred to PVDF membranes with iBlot^™ 2 transfer device (Thermo Fisher IB21001) using P0 protocol, and probed with indicated primary antibodies. Bound antibodies were detected with LI-COR IRDye ^® 680/800CW anti-rabbit/mouse secondary antibodies. Primary antibodies listed in the Supplementary Table 5.

Spisak S., Chen D., Likasitwatanakul P., Doan P., Li Z., Bala P., Vizkeleti L., Tisza V., De Silva P., Giannakis M., Wolpin B., Qi J, & Sethi N.S. (2024). Utilizing a dual endogenous reporter system to identify functional regulators of aberrant stem cell and differentiation activity in colorectal cancer. bioRxiv.

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