Cells were seeded into 35-mm glass bottom dishes (MatTek). Images stacks were acquired every 30 s using an Olympus SpinSR confocal microscope comprising Olympus IX83 stand, Olympus 60×1.5 NA UPLAPO objective lens, Yokogawa CSU-W1 scanhead, and Hamamatsu Orca Fusion camera. Images were acquired with a 2 × 2 camera bin, giving a pixel size of 108 nm. Laser excitation and emission filters for the GFP and RFP channels were 488 nm (ex) 525/50 nm (em) and 561 nm (ex) 617/73 nm (em), respectively. The image acquisition software was Olympus cellSens v4.1.
Spinsr confocal microscope
Manufactured by Olympus
The SpinSR confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It utilizes spinning-disk confocal technology to provide high-speed, high-resolution imaging capabilities. The core function of the SpinSR is to capture detailed, optical sections of samples, enabling researchers to visualize and analyze intricate cellular and sub-cellular structures with precision.
Automatically generated - may contain errors
2 protocols using spinsr confocal microscope
1
Live-cell Imaging Using Confocal Microscopy
2
Intracellular Iron Quantification Using FeRhoNox-1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Intracellular Fe2+ accumulation was assessed using the FeRhoNox-1 fluorescent probe (Maokang Biotechnology). HCC1954 cells were treated with DMSO or 10 μM ML-SI1 for 48 h. Subsequently, the cells were incubated at 37 °C for additional 30 min in culture medium containing 5 μM FeRhoNox-1 and 1 μM LysoTracker Green (Thermo Fisher Scientific). Prior to imaging, the cells were rinsed twice with warm-PBS. All images were captured using an Olympus SpinSR confocal microscope, employing lasers with wavelengths of 561 nm and 488 nm to excite FeRhoNox-1 and LysoTracker Green respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!