Horseradish peroxidase conjugated goat anti mouse and anti rabbit secondary antibodies

The rabbit polyclonal anti-cGAS K491me antibody was produced by Boer Biotechnology (Chengdu, China). The mouse cGAS K491me peptide was synthesized and injected into rabbits. An affinity column with bound nonmodified peptide was used to collect and purify rabbit serum, excluding antibodies targeting nonmethylated cGAS. The antibody was then purified using an affinity column linked with bound cGAS K491me peptide. A rabbit polyclonal antibody recognizing human cGAS K506me was produced following the same protocol.
Antibodies recognizing cGAS, Tubulin, GST, His, SET1A, SET1B, SET7, MLL1, MLL3, γH2A.X and SMYD3 were obtained from Cell Signaling Technology. Antibodies recognizing RIOX2 were purchased from Atlas Antibodies. Antibodies recognizing RIOX1, ASH1L, PARP1, MLL2, SGF29, Timeless, NSD1, NSD2, H2A.X, CD11b, CD11a, CD34, Thy-1, and SMYD2 were purchased from Abcam. The antibody against Flag, anti-Flag M2 agarose beads, streptavidin-conjugated agarose beads, biotin, bovine serum albumin and poly(ADP-ribose) glycohydrolase (PARG) recombinant protein were purchased from Sigma. Horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit secondary antibodies were purchased from Thermo Fisher Scientific. biotin (terminal)-PAR Polymer was purchased from Trevigen.

Rabbit monoclonal antibodies against FoxM1 (5436S; 1:1000) and STMN1 (13655S; 1:1000) for WB were obtained from Cell Signaling Technology (Cell Signaling Technology, Beverly, MA, USA). Mouse monoclonal antibodies against FoxM1 for IHC (sc-376471; 1:50) and ChIP assay were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Mouse monoclonal antibody against β-actin (A5316; 1:2000) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit polyclonal antibody against α-Tubulin (11224-1-AP; 1:200) for immunofluorescence was purchased from Proteintech (Wuhan, Hubei, China). Horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit secondary antibodies were obtained from Thermo Fisher Scientific. Lipofectamine Reagent 2000 transfection reagent was obtained from Invitrogen (Waltham, MA, USA).