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Fitc conjugated mouse anti chicken cd4 antibody

Manufactured by Southern Biotech
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Sourced in United States

The (FITC)-conjugated mouse anti-chicken CD4 antibody is a laboratory reagent used for the identification and analysis of chicken CD4-positive cells. It is designed to bind specifically to the CD4 receptor expressed on the surface of certain immune cells, such as T helper cells. The fluorescein isothiocyanate (FITC) label allows for the detection and visualization of the antibody-antigen complex using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Pe conjugated mouse anti chicken cd3 antibody, by Southern Biotech (2 mentions) Fitc conjugated mouse anti chicken cd8α antibody, by Southern Biotech (2 mentions) Facsc 2000, by BD (2 mentions)

Close spelling variants of this product

FITC-conjugated mouse anti-chicken CD4 Mouse anti-chicken CD4-FITC CD4-FITC Anti-CD4

2 protocols using fitc conjugated mouse anti chicken cd4 antibody

1

Chicken Antibody and T-cell Response Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 0 and 5 dpi, the serum antibodies of chickens were detected by enzyme-linked immunosorbent assay (ELISA) as described previously (56 (link)). All samples were analyzed in triplicate.
To measure the percentages of CD4+ and CD8+ cells among CD3+ cells, peripheral blood lymphocytes (PBLs) were incubated with phycoerythrin (PE)-conjugated mouse anti-chicken CD3 antibody (Southern Biotech, USA) and fluorescein isothiocyanate (FITC)-conjugated mouse anti-chicken CD4 antibody (Southern Biotech) or FITC-conjugated mouse anti-chicken CD8α antibody (Southern Biotech) at 4°C for 1 h. After being washed with PBS, the PBLs were analyzed by fluorescence-activated cell sorter (FACS; FACSC 2000, BD).
Zhao N., Ming S., Sun L., Wang B., Li H., Zhang X, & Zhao X. (2021). Identification and Characterization of Eimeria tenella Microneme Protein (EtMIC8). Microbiology Spectrum, 9(1), 10.1128/spectrum.00228-21.
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2

Chicken Antibody and T-cell Response Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 0 and 5 dpi, the serum antibodies of chickens were detected by enzyme-linked immunosorbent assay (ELISA) as described previously (56 (link)). All samples were analyzed in triplicate.
To measure the percentages of CD4+ and CD8+ cells among CD3+ cells, peripheral blood lymphocytes (PBLs) were incubated with phycoerythrin (PE)-conjugated mouse anti-chicken CD3 antibody (Southern Biotech, USA) and fluorescein isothiocyanate (FITC)-conjugated mouse anti-chicken CD4 antibody (Southern Biotech) or FITC-conjugated mouse anti-chicken CD8α antibody (Southern Biotech) at 4°C for 1 h. After being washed with PBS, the PBLs were analyzed by fluorescence-activated cell sorter (FACS; FACSC 2000, BD).
Zhao N., Ming S., Sun L., Wang B., Li H., Zhang X, & Zhao X. (2021). Identification and Characterization of Eimeria tenella Microneme Protein (EtMIC8). Microbiology Spectrum, 9(1), 10.1128/spectrum.00228-21.
+ Open protocol
+ Expand

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