Oxazepam

Diazepam, norDiazepam, delorazepam, 7-aminoclonazepam, 7-aminoflunitrazepam, alprazolam, α-hydroxyalprazolam, 2-hydroxyethylflurazepam, bromazepam, clonazolam, desalkylflurazepam, etizolam, flurazepam, lorazepam, lormetazepam, midazolam, phenazepam, diclazepam, Diazepam-D5, 7-aminoclonazepam-D4, and alprazolam-D5 were obtained from Cerilliant Corporation (Merck, Milan, Italy); flualprazolam, flubromazolam, and flubromazepam were purchased from Cayman Chemical Company (LGC standards, Milan, Italy); oxazepam, triazolam, and temazepam salts were purchased from Formenti (Formenti SPA, Milan, Italy); bentazepam methanolic solution, at a concentration of 0.1 mg/mL, was kindly donated from Italian Early Warning System, Italian Institute of Health (ISS).
Formic acid for mass spectrometry was obtained from Merck (Milan, Italy). High-performance liquid chromatography (HPLC) grade methanol and acetonitrile were purchased from Carlo Erba SRL (Milan, Italy). N-Hexane and ethyl acetate were purchased from Merck (Milan, Italy). β-glucuronidase from Escherichia Coli was purchased from Merck (Milan, Italy).

Prestwick FDA Approved Chemical Library was provided by MSU Assay Development and Drug Repurposing Core (ADDRC). R-(−)-apomorphine hydrochloride, raloxifene hydrochloride, fosinopril, nifedipine, nisoldipine, idebenone, itraconazole, prednicarbate, carmofur, diazepam, flurazepam, temazepam, prazepam, nitrazepam, nimetazepam, nordiazepam and oxazepam were from Cayman Chemical (Ann Arbor, MI). Devazepide was purchased from Santa Cruz Biotechnology (Dallas, Texas). Fluorescein diacetate, propidium iodide, clofazimine, hexachlorophene, hydralazine hydrochloride, thioflavin S and Amicon Centrifugal Filter Unit were purchased from Sigma Aldrich (St. Louis, MO). Gibco Dulbecco’s Modified Eagle Medium (DMEM), HyClone™ Fetal Bovine Serum, Optical Adhesive Film and FITC-Annexin V were purchased from Thermo Fisher Scientific (Waltham, MA). All other chemicals for common buffers and solutions were from Sigma Aldrich.