Bx 41 biological microscope

The tissues from the right liver lobe of rats were obtained and embedded into paraffin. Sections (4 μm thick) were prepared, dewaxed, stained with hematoxylin for 5 min, and rinsed with water. Sections were treated with hydrochloric acid/ethanol for 30 s for differentiation and then soaked in clean water for 15 min. Sections were stained with eosin for 2 min, dehydrated, and then mounted using neutral gum. The portal and lobular areas were observed under a BX41 biological microscope (OLYMPUS Corp., Tokyo, Japan). Based on the scores obtained using the Ishak Standards, liver pathology was divided into four degrees: 0 (-), normal liver tissue with no obvious degeneration, necrosis, and inflammatory cell infiltration; 1 (+), normal hepatic lobules with apparent cloudy swelling, ballooning degeneration or fatty degeneration, and scattered punctate necrosis; 2 (+ +), unclear lobular structure and obvious focal necrosis with inflammatory cell infiltration; 3 (+ + +), unclear lobular structure and obvious necrosis associated with inflammatory cell infiltration.