Ab196579
Ab196579 is a primary antibody that can be used to detect the target protein in laboratory experiments. The core function of this product is to specifically bind to and identify the target protein within a sample.
Lab products found in correlation
2 protocols using ab196579
Immunofluorescence Microscopy of Cellular Structures
Focal Adhesion Dynamics in hMSC Spheroids
and distribution of focal adhesions in hMSC-nanofunctionalized microparticle
spheroids were qualitatively assessed via confocal fluorescence microscopy
analysis. After 4 and 24 h in culture, the spheroids were fixed with
warm 10% FA in DPBS solution for 20 min and permeabilized using a
0.1% v/v solution of Triton-X100 (Sigma-Aldrich) in Milli-Q water
for 30 min. After this, an unspecific binding blocking step of 1 h
was performed in a casein-based blocking serum (CAS-Block Histochemical
Reagent; Thermo Fisher Scientific). The spheroids were then incubated
overnight with an Alexa Fluor 647-conjugated monoclonal antivinculin
antibody (ab196579, Abcam) solution in a 0.1% w/v bovine serum albumin
(BSA; VWR) solution in phosphate-buffered saline (PBS) with a 1:100
dilution to visualize focal adhesions. At the same time, cell nuclei
were counterstained overnight using diamidino-2-phenylindole dihydrochloride
(DAPI; Sigma-Aldrich) at a dilution of 1:200 in 0.1% BSA in PBS. The
imaging was performed using a confocal laser scanning electron microscope
(TCS SP8 STED, Leica Microsystems), acquiring z-stacks of 4 μm
thickness for each image.
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