Log In Sign up
The largest database of trusted experimental protocols

Tubulin

Manufactured by BD
Visit Supplier

Tubulin is a core structural protein found in eukaryotic cells. It is the main component of microtubules, which are essential for various cellular processes such as cell division, intracellular transport, and cell motility.

Automatically generated - may contain errors

Lab products found in correlation

Protease and phosphatase cocktail, by Thermo Fisher Scientific (1 mentions) Non phospho β catenin, by Cell Signaling Technology (1 mentions) Desmoglein 2, by Santa Cruz Biotechnology (1 mentions) C fos, by Santa Cruz Biotechnology (1 mentions) Gsk3α, by Cell Signaling Technology (1 mentions) Pierce bca assay kit, by Thermo Fisher Scientific (1 mentions) Connexin 43, by Merck Group (1 mentions) Timp3, by Abcam (1 mentions) Desmocollin 1, by Santa Cruz Biotechnology (1 mentions) Phospho gsk3α, by Cell Signaling Technology (1 mentions) Connexin 26, by Santa Cruz Biotechnology (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) β catenin, by BD (1 mentions) Anti 16e7, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

2 protocols using tubulin

1

Protein Expression Analysis of Cell Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols
After desired treatments, cells were lysed with RIPA buffer spiked with a fresh protease and phosphatase cocktail (Thermo Scientific, #78442) and sonicated. Protein concentrations were quantified using the Pierce BCA assay kit (Thermo Fisher, #23225). 80–120μg of protein for each sample was loaded onto SDS-PAGE gels, and then transferred onto PVDF membranes. The blots were incubated with the following antibodies: desmocollin 1 (sc-398590, RRID: AB_2894905), desmoglein 2 (sc-80663, RRID: AB_2093438), plakophilin (sc-33636, RRID: AB_2164139), connexin 26 (sc-7261, RRID: AB_2110895) and cFOS (sc-52, RRID: AB_2106783) from Santa Cruz; ER-α (#8644, RRID: AB_2617128), HA (#3724, RRID: AB_1549585), Non-phospho-β-catenin (#19807, RRID: AB_2650576), Histone H3 (#4499, RRID: AB_10544537), AIF (#5318, RRID: AB_10634755), GSK3β (Ser9, #5558, RRID: AB_10013750), phospho-GSK3α (Ser21, #9316, RRID: AB_659836), GSK3β (#12456, RRID: AB_2636978) and GSK3α (#4337, RRID: AB_10859910) from Cell Signaling Technology; β-catenin (#610154, RRID: AB_397555) from BD; Tubulin (T6557, RRID: AB_477584) and connexin 43 (C6219, RRID: AB_476857) from Sigma Aldrich; and TIMP3 (ab39184, RRID: AB_2204971) from Abcam.
Li Z., Wu Y., Yates M.E., Tasdemir N., Bahreini A., Chen J., Levine K.M., Priedigkeit N.M., Nasrazadani A., Ali S., Buluwela L., Arnesen S., Gertz J., Richer J.K., Troness B., El-Ashry D., Zhang Q., Gerratana L., Zhang Y., Cristofanilli M., Montanez M.A., Sundd P., Wallace C.T., Watkins S.C., Fumagalli C., Guerini-Rocco E., Zhu L., Tseng G.C., Wagle N., Carroll J.S., Jank P., Denkert C., Karsten M.M., Blohmer J.U., Park B.H., Lucas P.C., Atkinson J.M., Lee A.V, & Oesterreich S. (2022). Hotspot ESR1 mutations are multimodal and contextual modulators of breast cancer metastasis. Cancer research, 82(7), 1321-1339.
+ Open protocol
+ Expand
2

Protein Expression Analysis in Keratinocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
SDS-lysed keratinocyte cell lysates were equalized for protein concentration using BioRad reagents. Equal amounts of protein-normalized samples were loaded onto SDS-acrylamide gels, electrophoresed, and transferred onto PVDF membranes. Blots were blocked in 0.05% tween-20/5% Non-fat milk in Tris-buffered saline and probed with the indicated antibodies to GAPDH (#368 from Cell Signaling), Tubulin (#T9026 from BD Biosciences), anti-16E7 (a mix of both monoclonal antibody clones 8C9 and EDV7 from Santa Cruz Biotechnology), and antibodies from ThermoFischer to actin (MS-1295-P1) and p53 (MA1-19055). Anti-AU1 tag monoclonal antibody was a gift of Richard Schlegel (Georgetown University). Anti-16E6 MAb 6G6 was a kind gift of Johannes Schweitzer (Arbor Vita Corp.); lysates for 16E6 western blots were prepared from Igepal-lysed cells as described [45 (link)].
Brimer N, & Vande Pol S. (2022). Human papillomavirus type 16 E6 induces cell competition. PLoS Pathogens, 18(3), e1010431.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!