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Mouse anti sc35 s4045

Manufactured by Merck Group

Mouse anti-SC35 (S4045) is a primary antibody produced in mice that recognizes the SC35 protein. SC35 is a non-snRNP splicing factor involved in pre-mRNA splicing. This antibody can be used for applications such as immunohistochemistry, immunocytochemistry, and Western blotting.

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2 protocols using mouse anti sc35 s4045

1

Antibody Preparation and Characterization

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Rabbit anti–human Rab18 (Ozeki et al., 2005 (link)) and rabbit anti–human TIP47 antibodies (Ohsaki et al., 2006 (link)) were prepared as described. Rabbit anti–human PML (Xu et al., 2005 (link)) and rabbit anti-CCTα (Gehrig et al., 2008 (link)) were donated by K.-S. Chang (The University of Texas MD Anderson Cancer Center, Houston, TX) and N. Ridgway (Dalhousie University, Halifax, Nova Scotia), respectively. Mouse anti–human lamin A/C (4C11; Cell Signaling), rabbit anti-coilin (H-300; Santa Cruz Biotechnology, Inc.), mouse anti-SC35 (S4045), mouse anti-FLAG (M2; Sigma-Aldrich), and mouse anti–human perilipin2 (AP125; Progen) antibodies were obtained from the respective suppliers. Cy3-conjugated donkey anti–rabbit IgG, Cy3-conjugated donkey anti–mouse IgG, Alexa Fluor 647–conjugated donkey anti–rabbit IgG (Jackson ImmunoResearch Laboratories), and Alexa Fluor 594 FluoroNanogold-conjugated anti–rabbit IgG (Nanoprobes) antibodies were also purchased.
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2

Comprehensive Antibody Panel for Cellular Analysis

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Goat anti-TIAR (C-18; sc-1749), goat anti-TIA1 (sc-1671), goat anti-eIF3B (eIF3eta; sc-16377), rabbit anti-p38 MAPK (sc-535), and mouse anti-G3BP (sc-81940) are from Santa Cruz; mouse anti-phospho-RPOL2A (Ser2; H5; ab24758), mouse anti-U1A (ab55751), rat anti-U1C (ab122901), rabbit anti-H3 (ab1791), rabbit anti-PRP19 (ab27692), rabbit anti-PRPF3 (ab187535), and rabbit anti-U2AF65 (ab37530) are from Abcam; rabbit anti-phospho-H3 (Ser10; #9701), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182; #9211), and rabbit anti-phospho-SF3B1 (Thr313; #25009) are from Cell Signaling Technology; mouse anti-U1-70K (05-1588) and mouse anti-puromycin (MABE343) are from Millipore; mouse anti-SC35 (S4045), rabbit anti-SON (HPA031755), and rabbit anti-CASC3 (HPA050262) are from Sigma-Aldrich; and rabbit anti-Calnexin polyclonal antibody (ADI-SPA-860-F) is from Enzo.
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