Following pupil dilation using 1% tropicamide solution (Minims, Bausch + Lomb, Surrey, UK), mice were placed on a heated platform to regulate their body temperature and prevent cold-induced cataracts. The cornea was kept moist using a lubricating eye gel (Polygel®; Alcon Laboratories, Frenchs Forrest, NSW, Australia). High-resolution fundus images were acquired using a Micron IV imaging system (Phoenix Research Labs, Pleasanton, CA, USA) at baseline prior to EAU induction and weekly thereafter for a period of 12 weeks. A set of five fundus images was collected for each eye (Figure 1 a); one with the optic nerve head (ONH) positioned in the centre, one each with the ONH oriented towards the left and right side and two with the ONH oriented towards the bottom (exposing the superior region) and top (exposing the inferior region). From the fundus images, the condition of the optic disc, retinal blood vessels, retinal tissues and structural damage were assessed using the following protocol. All assessments were performed by an unmasked researcher with results validated by a masked observer.
Polygel
Manufactured by Alcon
Sourced in Australia
Polygel is a specialized laboratory equipment designed for the preparation and handling of gel-based samples. It provides a controlled environment for the gelation and polymerization of various materials. The core function of Polygel is to facilitate the formation and analysis of gel-based substances, which are commonly used in scientific research and various industrial applications.
Automatically generated - may contain errors
2 protocols using polygel
1
Detailed Mouse Fundus Imaging Protocol
2
In-vivo Retinal Layer Analysis using OCT
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The optical coherence tomography (OCT) imaging technique was used to obtain information on in vivo retinal layers and choroid structure. Spectral-domain optical coherence tomography (SD-OCT, Micron IV; Phoenix Research Laboratories, Pleasanton, CA, USA) was employed. This procedure was executed immediately after ERG recordings under anesthesia and pupil dilation. 5 Rats were placed on a 378C heating pad to maintain their body temperature and to prevent the development of cold cataracts. Dilated eyes were covered with Poly Gel (3 mg/g Carbomer; Alcon, NSW, Australia) and the retina was visualized by contacting the OCT lens to the gel. StreamPix 6 software, version 7.2.4.2 (Phoenix Research Laboratories) was used in image acquisition. The SD-OCT ultra-broadband (160 nm) light source was used to obtain 1024 pixels per Ascan and 10 frames per horizontal B-scan with 2-lm axial resolution. Images were acquired 2 mm from the optic nerve in the dorsal retina. Images were analyzed using InSight software, version 1.1.5207 (Phoenix Research Laboratories) to calculate the thickness of retinal layers and choroid. The choroid was measured from the hyperreflective Bruch's membrane to the choroidal vessels and the sclera intersection. The outer nuclear layer was measured from the RPE hyperreflective line to the outer plexiform layer (OPL) interface.
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