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Ghost viability dye bv540

Manufactured by Cytek Biosciences
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The Ghost viability dye BV540 is a fluorescent dye used in flow cytometry applications to distinguish viable cells from non-viable cells. The dye binds to cellular proteins, emitting a fluorescent signal that can be detected by flow cytometers.

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Lab products found in correlation

Cd16 32, by Cytek Biosciences (2 mentions) Fixation buffer, by Cytek Biosciences (2 mentions)

Close spelling variants of this product

Ghost viability dye Ghost Dye Viability dye

2 protocols using ghost viability dye bv540

1

Multiparameter Flow Cytometry Staining

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Cells were stained in the presence of 1:500 Fc block (CD16/32, Tonbo) and antibodies diluted 1:200 in FACs buffer (PBS+2.5% FBS) for 45 minutes in the dark at 4°C. Ghost viability dye BV540 (Tonbo) was used to exclude dead cells at 1:500. Cells were fixed in 2% PFA or fixation buffer (Tonbo) for 10–15 at room temperature in the dark prior to data acquisition. Cells were acquired within 24 h using a Cytek Aurora.
Ben-Artzi H., Zeelon E., Gorecki M, & Panet A. (1992). Double-stranded RNA-dependent RNase activity associated with human immunodeficiency virus type 1 reverse transcriptase.. Proceedings of the National Academy of Sciences of the United States of America, 89(3), 927-931.
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2

Multiparameter Flow Cytometry Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained in the presence of 1:500 Fc block (CD16/32, Tonbo) and antibodies diluted 1:200 in FACs buffer (PBS+2.5% FBS) for 45 minutes in the dark at 4°C. Ghost viability dye BV540 (Tonbo) was used to exclude dead cells at 1:500. Cells were fixed in 2% PFA or fixation buffer (Tonbo) for 10–15 at room temperature in the dark prior to data acquisition. Cells were acquired within 24 h using a Cytek Aurora.
Patterson M.T., Burrack A.L., Xu Y., Hickok G.H., Schmiechen Z.C., Becker S., Cruz-Hinojoza E., Schrank P.R., Kennedy A.E., Firulyova M.M., Miller E.A., Zaitsev K., Williams J.W, & Stromnes I.M. (2023). Tumor-specific CD4 T cells instruct monocyte fate in pancreatic ductal adenocarcinoma. Cell reports, 42(7), 112732.
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