The human ovarian cancer cell lines SKOV3 (ATCC® HTB-77™) and Caov-3 (ATCC® HTB-75™) were purchased from the American Type Culture Collection (Rockville, MD, USA). A2780 (ECACC 93112519) was obtained from the European Collection of Authenticated Cell Cultures. Patricia Donahoe (Massachusetts General Hospital, Boston, MA, USA) provided the human IGROV-1, OVCAR5, and OVCAR8 ovarian cancer cell lines, which have been authenticated and are free of mycoplasma contamination as verified by the MycoAlert Mycoplasma Detection Kit from Cambrex (Rockland, ME, USA). All these cell lines were maintained in RPMI 1640 (GE Healthcare Life Sciences, Logan, UT, USA) medium supplemented with 10% FBS (MilliporeSigma, Burlington, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator containing 5% CO2 at 37°C. The cells were resuspended with 0.05% trypsin-EDTA (Life Technologies Corporation, Grand Island, NY, USA) before subculturing.
A2780 ecacc 93112519
Manufactured by European Collection of Authenticated Cell Cultures
A2780 (ECACC 93112519) is a human ovarian carcinoma cell line derived from an untreated patient. This cell line is available from the European Collection of Authenticated Cell Cultures (ECACC).
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Rpmi 1640, by GE Healthcare (2 mentions)
Caov 3 atcc htb 75, by American Type Culture Collection (2 mentions)
Mycoalert mycoplasma detection kit, by Cambrex (1 mentions)
Atcc htb 77, by American Type Culture Collection (1 mentions)
Skov3 atcc htb 77, by American Type Culture Collection (1 mentions)
2 protocols using a2780 ecacc 93112519
1
Ovarian Cancer Cell Line Cultivation
2
Culturing Ovarian Cancer Cell Lines
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Human ovarian cancer cell lines Skov3 (ATCC® HTB-77™), and Caov-3 (ATCC® HTB-75™) were purchased from the American Type Culture Collection (Rockville, MD). A2780 (ECACC 93112519) was obtained from the European Collection of Authenticated Cell Cultures. Dr. Patricia Donahoe (Massachusetts General Hospital, Boston, MA) provided the human IGROV-1, OVCAR5, and OVCAR8 ovarian cancer cell lines. These cell lines were maintained in RPMI 1640 (GE Healthcare Life Sciences, Logan, Utah, USA) medium supplemented with 10% FBS (MilliporeSigma, Burlington, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator containing 5% CO 2 at 37°C. Cells were resuspended with 0.05% trypsin-EDTA (Life Technologies Corporation, NY, USA) before subculture.
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