Statistical software version 1

This study set out to determine the influence of PPV on cell migration and the expression of VEGF B, VEGF R1, VEGF R2, and FAK in a triple negative breast cancer cell (MDA-MB-231), alveolar adenocarcinoma cells (A549), and prostate adenocarcinoma cells (DU145). Three independent experimental repeats were conducted for all the techniques conducted where the mean and the standard deviation were calculated. Means were illustrated by using bar charts, and standard deviations were shown with errors bars. A p-value < 0.05 was calculated using the Student t-test for statistical significance and was indicated by an asterisk (*) using Jamovi statistical software version 1.6 (The Jamovi project (2021) (Sydney, Australia). Image J software developed by the National Institutes of Health (Bethesda, MD, USA) was used to quantify the scratch assay.

Three independent experiments were conducted for all techniques performed, where the mean and the standard deviation were calculated. Means are illustrated by using bar charts and standard deviations are shown with errors bars. A p-value < 0.05 calculated by means of the Student t-test was used for statistical significance and is indicated by an asterisk (*) using the Jamovi statistical software version 1.6 (The Jamovi project (2021) (Sydney, Australia). The fluorescent intensity of at least 100 cells was evaluated per condition using Image J software developed by the National Institutes of Health (Bethesda, Maryland, United States of America). Flow cytometry analysis involved at least 10,000 events in each sample and the data were analysed using FlowJo™ Software Version 10 (Becton, Dickinson, and Company, 2019 (Ashland, Oregon, United States of America)).