Phylogenetic relationships were investigated by amplifying and sequencing the housekeeping genes translation elongation factor 1-α (TEF), calmodulin (CAL) and βtubulin (TUB). Fragments of TEF gene were amplified using the primer pair EF1 and EF2 2000) and the TUB gene were amplified using the primers Bt2a and Bt2b (Glass & Donaldson 1995) . Before sequencing, PCR products were purified with the enzymatic mixture EXO/FastAP (Exonuclease I, FastAP thermosensitive alkaline phosphatase, Thermo Scientific). Sequence reactions were performed using a BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit for both strands, after which they were purified by gel filtration through Sephadex G-50 (5%) (Sigma Aldrich), before they were analyzed on the 3730 DNA Analyzer (Applied Biosystems).
Bigdye terminator v3.1 cycle sequencing ready reaction kit
The BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit is a reagent kit used for DNA sequencing. It contains the necessary components for performing cycle sequencing reactions, including DNA polymerase, dNTPs, and fluorescently labeled dideoxynucleotides. The kit is designed to enable efficient and accurate DNA sequencing.
2 protocols using bigdye terminator v3.1 cycle sequencing ready reaction kit
Molecular Phylogeny of Fungal Strains
Phylogenetic relationships were investigated by amplifying and sequencing the housekeeping genes translation elongation factor 1-α (TEF), calmodulin (CAL) and βtubulin (TUB). Fragments of TEF gene were amplified using the primer pair EF1 and EF2 2000) and the TUB gene were amplified using the primers Bt2a and Bt2b (Glass & Donaldson 1995) . Before sequencing, PCR products were purified with the enzymatic mixture EXO/FastAP (Exonuclease I, FastAP thermosensitive alkaline phosphatase, Thermo Scientific). Sequence reactions were performed using a BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit for both strands, after which they were purified by gel filtration through Sephadex G-50 (5%) (Sigma Aldrich), before they were analyzed on the 3730 DNA Analyzer (Applied Biosystems).
Molecular Phylogeny of Fungal Strains
Phylogenetic relationships were investigated by amplifying and sequencing the housekeeping genes translation elongation factor 1-α (TEF), calmodulin (CAL) and βtubulin (TUB). Fragments of TEF gene were amplified using the primer pair EF1 and EF2 2000) and the TUB gene were amplified using the primers Bt2a and Bt2b (Glass & Donaldson 1995) . Before sequencing, PCR products were purified with the enzymatic mixture EXO/FastAP (Exonuclease I, FastAP thermosensitive alkaline phosphatase, Thermo Scientific). Sequence reactions were performed using a BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit for both strands, after which they were purified by gel filtration through Sephadex G-50 (5%) (Sigma Aldrich), before they were analyzed on the 3730 DNA Analyzer (Applied Biosystems).
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