U46619 and I-BOP, selective TP agonists, and SQ29548, a selective TP antagonist, were purchased from Cayman Chemical (Ann Arbor, MI, USA). Prostaglandin E2 (PGE2), elastase type II-A, trypsin inhibitor type I-S, bovine serum albumin V, penicillin-streptomycin solution, DMEM, and HBSS were purchased from Sigma-Aldrich (Tokyo, Japan). Collagenase II was purchased from the Worthington Biochemical Corp. (Lakewood, NJ, U.S.A.). Collagenase/dispase was purchased from Roche Diagnostics (Tokyo, Japan). Fetal bovine serum was purchased from Invitrogen (Tokyo, Japan), and 3% buffered formalin was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
Bovine serum albumin 5
Manufactured by Merck Group
Sourced in Japan
Bovine serum albumin V is a purified protein derived from bovine serum. It is commonly used as a stabilizing agent and blocking reagent in various laboratory techniques.
Automatically generated - may contain errors
Lab products found in correlation
Collagenase dispase, by Roche (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Hanks balanced salt solution (hbss), by Merck Group (1 mentions)
U46619, by Cayman Chemical (1 mentions)
Collagenase 2, by Worthington (1 mentions)
Penicillin streptomycin solution, by Merck Group (1 mentions)
Trypsin inhibitor type 1 s, by Merck Group (1 mentions)
I bop, by Cayman Chemical (1 mentions)
Sq29548, by Cayman Chemical (1 mentions)
Protease inhibitor, by Roche (1 mentions)
Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions)
Imagequant 800 system, by Cytiva (1 mentions)
2 protocols using bovine serum albumin 5
1
Reagents for Vascular Smooth Muscle Cell Studies
2
Quantifying Innate Immune Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total protein lysates from MDDC and cDC cultured for 1 h in the presence of media or individual or combined Poly I:C and 2´3´-di AM(PS) agonists were obtained using RIPA buffer containing 1% phosphatase and protease inhibitors (Roche Diagnostics). Subsequently, protein lysates were resolved in a 10% agarose gel with SDS and transferred to a nitrocellulose membrane (Fisher Scientific). Membranes were blocked in 5% bovine serum albumin v (Sigma-Aldrich) in Tris buffered saline and incubated overnight with 1:100 dilution of primary anti-TBK1, anti-IRF3 or anti-GAPDH antibodies (Table 1). Then, membranes were incubated for 1 h with the appropriate anti-rabbit or anti-mouse secondary antibodies (Table 1). Protein band intensity was quantified by analyzing chemiluminescence detected using an ImageQuant 800 system (Amersham).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!