Cd27 apc cy7

Manufactured by Thermo Fisher Scientific

CD27-APC/Cy7 is a fluorochrome-conjugated antibody that binds to the CD27 cell surface antigen. CD27 is a co-stimulatory receptor expressed on T cells, B cells, and natural killer cells. This product can be used for the identification and characterization of CD27-positive cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

CD27-APC (18 citations) APC-Cy7 (32 citations)

2 protocols using cd27 apc cy7

Phenotypic Characterization of Murine Hematopoietic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow/hematopoietic stem cells were extracted from femurs and tibia by flushing with PBS + 2% FBS. Peripheral blood cells/granulocytes were collected via the tail vein in EDTA. Red blood cells were lysed using 1x ACK Lysis Buffer. Cells were then stained for analysis by flow cytometry with the following surface antibodies purchased from Biolegend at 1:200 unless otherwise indicated: CD34-eFlour660 (1:50, 50-0341-80, eBioscience), Sca-1-AF700 (1:100, 108141), Ter119-PE/Cy5 (116209), ckit/CD117-PE/Cy7 (25-1171-81, eBioscience), CD150/SLAM-PerCP-eFlour710 (46-1502-82, eBioscience), CD11b-APC (101212), Gr1-AF700 (108422), CD45-APC/Cy7 (103116), NK1.1-PE (108707), and CD27-APC/Cy7 (124226). Flow cytometry analysis was performed using the BD LSRII.

Hohsfield L.A., Najafi A.R., Ghorbanian Y., Soni N., Hingco E.E., Kim S.J., Jue A.D., Swarup V., Inlay M.A, & Green K.N. (2020). Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS. Journal of Neuroinflammation, 17, 279.

+ Open protocol

+ Expand

Murine Hematopoietic Stem Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow/Hematopoietic stem cells were extracted from femurs and tibia by ushing with PBS + 2% FBS. Peripheral blood cells/granulocytes were collected via the tail vein in EDTA. Red blood cells were lysed using 1x ACK Lysis Buffer. Cells were then stained for analysis by ow cytometry with the following surface antibodies purchased from Biolegend at 1:200 unless otherwise indicated: CD34-eFlour660 (1:50, 50-0341-80, eBioscience), Sca-1-AF700 (1:100, 108141),, Ter119-PE/Cy5 (116209), ckit/CD117-PE/Cy7 (25-1171-81, eBioscience), CD150/SLAM-PerCP-eFlour710 (46-1502-82, eBioscience), CD11b-APC (101212), Gr1-AF700 (108422), CD45-APC/Cy7 (103116), NK1.1-PE (108707) and CD27-APC/Cy7 (124226). Flow cytometry analysis was performed using the BD LSRII.

Hohsfield L.A., Najafi A.R., Ghorbanian Y., Soni N., Hingco E., Kim S.J., Jue A.D., Swarup V., Inlay M.A., & Green K.N. (2020). Effects of long-term and brain-wide colonization of peripheral bone-marrow derived myeloid cells in the CNS.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!