Hylin a1 and RGD-hylin a1 were synthesized by Ango Biotechnology Co., Ltd. (Shanghai, China). Dimethyl Sulphoxide (DMSO) and Triton-100 were of analytical grade and were used without further purification. These reagents were purchased from Sigma-Aldrich (USA). 0.25% Tripsin was purchased from Wisent (Australia). The fetal bovine serum was purchased from Millipore (USA), and DMEM was purchased from Hyclone (USA). Annexin V FITC Apoptosis Detection Kit (Cat. NO. 556547) and Flow Cytometry Mitochondrial Membrane Potential Detection Kit (Cat. NO. 551302) were purchased from BD (USA). Cetyltrimethylammonium bromide (CTAB) was from Amresco. Poly acrylic acid (PAA, average molecular weight 240,000, 25 wt % solution in water) was from Alfa Aesar. NaCl, Na2HPO4.2H2O, KH2PO4, and KCl were all of analytical grade. The 10 mM phosphate-buffered saline (PBS) solutions (pH = 7.4) contained 0.01 M sodium phosphate, 0.137 M NaCl, 0.01 M Na2HPO4.2H2O, 0.002 M KH2PO4 and 0.0027 M KCl. Ultrapure water in the experiment was provided by Milli-Q Academic system (USA).
Na2hpo4.2h2o
Manufactured by Thermo Fisher Scientific
Sourced in India
Na2HPO4.2H2O is a chemical compound commonly known as disodium hydrogen phosphate dihydrate. It is a crystalline solid that is used as a buffer in various laboratory and industrial applications. The compound is soluble in water and has a pH-regulating effect, making it useful in maintaining specific pH levels in solutions.
Automatically generated - may contain errors
Lab products found in correlation
Kh2po4, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Cetyltrimethylammonium bromide ctab, by Avantor (1 mentions)
Polyacrylic acid paa, by Thermo Fisher Scientific (1 mentions)
Cm3050, by Leica (1 mentions)
Nah2po4.2h2o, by Thermo Fisher Scientific (1 mentions)
Bacto peptone, by Thermo Fisher Scientific (1 mentions)
Bacto yeast extract, by Thermo Fisher Scientific (1 mentions)
D maltose monohydrate, by Merck Group (1 mentions)
Kh2po4, by Carl Roth (1 mentions)
Filtropur, by Sarstedt (1 mentions)
10 cm cell culture dishes, by Greiner (1 mentions)
Cell culture water, by Merck Group (1 mentions)
Na2hpo4 2h2o, by Carl Roth (1 mentions)
4 protocols using na2hpo4.2h2o
1
Synthesis and Characterization of Hylin a1 Peptides
2
Graphene Oxide Synthesis and Erythropoietin Characterization
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Graphene oxide sample GO 1 was prepared using a modified Hummers method. 19 Graphene oxide sample GO 2 was purchased from Grupo Antolin (Spain). GO 3 was also prepared using a modified Hummers method. 21 EPO derived from human neutrophils (Athens Research and Technology, USA) with an activity of greater than 180 units per mg protein after lyophilization.
Hydrogen peroxide (30% aqueous solution), NaBr, NaH2PO4•2H2O and Na2HPO4•2H2O were purchased from Alfa Aesar and used directly without any further purification.
Hydrogen peroxide (30% aqueous solution), NaBr, NaH2PO4•2H2O and Na2HPO4•2H2O were purchased from Alfa Aesar and used directly without any further purification.
3
Mouse Brain Perfusion and Tissue Sectioning
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All experimental mice were transcardially perfused with ice-cold Phosphate Buffered Saline (PBS) containing NaH2PO4.2H2O (Cat# 13472-35-0, Fisher Scientific, India), Na2HPO4.2H2O (Cat# 10028-24-7, Fisher Scientific, India), NaCl (Cat# 7647-14-5, Fisher Scientific, India), pH—7.4, and were fixed with 4% paraformaldehyde (PFA) (Cat# GRM3660, HiMedia, India). The flow rate was maintained continuously at 10.2 mL/min using a peristaltic pump (Cat# RH-P110S-25, Ravel Hiteks PVT LTD, India). After perfusion, the tissues were subjected to post-fixation in 4% PFA for a day and preserved in 30% sucrose (with 0.02% sodium azide) (Cat# GRM134, Hi-Media, India) at 4 °C till further usage. Sucrose-treated brains were sectioned in a cryostat (Cat# CM3050s, Leica, India) with Internal chamber temperature (CT) maintained at -20 °C, and Object Temperature (OT) at − 22 °C throughout the procedure. 40 µm thick coronal sections were obtained on gelatin-coated slides (3% gelatin, Cat# GRM019, Hi-Media, India, and 0.5% Chromium potassium sulphate dodecahydrate, Cat# GRM3042, Hi-Media, India). These slides were stored at 4 °C until further usage.
4
D. discoideum Actin Dynamics Imaging
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Cells of D. discoideum strain AX2 (clone: EB27-3-4), expressing LimEdeltacc-GFP as an actin probe, were a gift from Günther Gerisch (MPI of Biochemistry, Martinsried). The cells were cultivated at 22°C in 10 cm cell culture dishes (Greiner Bio-one) with nutrient medium containing 10 μg/ml of G418 (Sigma-Aldrich). Nutrient medium consists of 7.15 g Bacto™ Yeast Extract (Thermo Fisher), 14.3 g Bacto™ Peptone (Thermo Fisher), 18.0 g D-(+)-maltose monohydrate (Sigma-Aldrich), 0.0486 g KH2PO4 (Roth), 0.616 g Na2HPO4 * 2H2O (Roth) in 1 L cell culture water (Sigma-Aldrich), was adjusted to pH 6.7, and subsequently filtered by the use of a 0.45 µm porous membrane (Filtropur; Sarstedt). The cells were split every 2–3 days in a ratio of 1:5–1:10, before the cell monolayers became confluent. For starvation, 5 * 106 cells were shaken overnight (60 turns/min) at 22°C in an Erlenmeyer flask (volume: 10 ml) with 3 ml phosphate buffer (17 mM; 2.0 g KH2PO4 and 0.356 g Na2HPO4 * 2H2O in 1 L cell culture water, pH 6.0) placed on a shaker with orbital motion (GFL 3015; Fisher Scientific) and subsequently used for microfluidic experiments.
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