Cells were cultured at a density of 100 × 104 cells in a 60-mm culture plate (Tarsons-960020). Four hours before the addition of the TLR3 ligand, the MyD88 inhibitor was added and incubated for 24 h. Cells were lysed using RIPA buffer, and gel electrophoresis was performed using acrylamide gel. Proteins were transferred to PVDF membranes and blotted with antibodies against interleukin 1 receptor-associated kinase 1 (IRAK1; Invitrogen-38-5600), phospho IRAK1–Thr209, (Invitrogen-PA5-38633), transforming growth factor beta-activated kinase 1 (TAK1; Invitrogen-700 113), phospho TAK1–Thr184/187 (Invitrogen-MA5-15073), TGF-beta-activated kinase 1 (TAB1; Invitrogen-PA5-28683), TNF receptor-associated factor 6 (TRAF6; Invitrogen-PA5-29622), and cyclin D1 (Invitrogen-AHF0082). The antibody against β-actin (Invitrogen-MA191399) was used as a loading control.
Ma5 15073
Manufactured by Thermo Fisher Scientific
The MA5-15073 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for a specific function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation or interpretation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ma5 15073
1
TLR3-Induced Signaling Pathway Analysis
2
TLR3 Signaling Pathway Regulation
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Cells were cultured at a density of 100x10 4 cells in 60 mm culture plate (Tarsons-960020). Four hours before the addition of TLR3 ligand, MyD88 inhibitor was added and incubated for 24 hours. Cells were lysed using RIPA buffer and gel electrophoresis was performed using acrylamide gel. Proteins were transferred to PVDF Membranes and blotted with antibodies against IRAK1 (Invitrogen-38-5600), phospho IRAK1-Thr209, (Invitrogen-PA5-38633), TAK1 (Invitrogen-700 113 ), phospho TAK1-Thr184/187 (Invitrogen-MA5-15073), TAB1
(Invitrogen-PA5-28683), TRAF-6 (Invitrogen-PA5-29622), and Cyclin D1 (Invitrogen-AHF0082). The antibody against β-actin (Invitrogen-MA191399) was used as a loading control.
(Invitrogen-PA5-28683), TRAF-6 (Invitrogen-PA5-29622), and Cyclin D1 (Invitrogen-AHF0082). The antibody against β-actin (Invitrogen-MA191399) was used as a loading control.
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