Lck 914

Manufactured by HACH

Sourced in Germany

The LCK 914 is a cuvette test for the determination of copper in water and wastewater samples. It is designed for use with Hach spectrophotometers and colorimeters.

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Lab products found in correlation

Lck 338, by HACH (3 mentions) Lck 341, by HACH (2 mentions) Lck 303, by HACH (2 mentions) Lck 304, by HACH (1 mentions) Hpx 87h column, by Aminex (1 mentions) Lck 514, by HACH (1 mentions) Gc 2010 plus, by Agilent Technologies (1 mentions) Lck 340, by HACH (1 mentions)

4 protocols using lck 914

Analytical Methods for Wastewater Characterization

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), using the commercial kit LCK 304 (Hach Lange®, Germany). At the end of each cycle total nitrogen (TN) determination was performed, using the commercial kits LCK 338 (Hach Lange®, Germany). Biomass concentration was measured as total suspended nitrogen (TSN), using the commercial kit LCK 304 (Hach Lange®, Germany).
Chemical oxygen demand (COD), nitrite and nitrate concentrations were determined using the commercial kit LCK 914, LCK 342 and LCK 339, respectively (Hach Lange®, Germany) .
The pH was manually controlled and measured by using a benchtop metre inoLabVR pH 7110 (WTW, Weilheim, Germany). The adjustment was performed by adding hydrochloric acid (HCl) with a syringe to the bioreactor until reaching the optimal pH.

Silva R.M., Fernandes A.M., Fiume F., Castro A.R., Machado R, & Pereira M.A. (2021). Sequencing batch airlift reactors (SBAR): a suitable technology for treatment and valorization of mineral oil wastewaters towards lipids production. Journal of hazardous materials, 409.

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Comprehensive Characterization of Anaerobic Samples

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All the samples were chemically characterized in terms of pH, TS, VS, carboxylic acids content and COD. TS and VS were analyzed according to standard methods (APHA, 2005) .
COD was determined by spectrophotometric analysis using the kits LCK 514 and LCK 914 (Hach-Lange). The concentration of total carboxylic acids and ethanol was determined using a gas chromatograph (Shimadzu GC-2010 Plus) equipped with an Agilent 19095F-123 capillary column (30 m length and 0.53 mm i.d.) followed by a flame ionization detector (FID). The carrier gas was nitrogen at 30 ml/min. The temperatures of the injector and the detector were 240 and 240 °C, respectively. Lactic acid concentration was analyzed by HPLC on a Dionex Ultimate 3000-LC system with an Aminex HPX-87H column coupled to a refractive index detector (RID). As mobile phase H2SO4 (4 mmol/l) was used. Methane and hydrogen production was analyzed using a gas chromatograph (SRI GC model 310) equipped with a Porapak Q column (182.88 cm length and 2.1 mm i.d.) followed by thermal conductivity detector (TCD). Nitrogen was used as carrier gas.

Fernando-Foncillas C., & Varrone C. (2021). Effect of reactor operating conditions on carboxylate production and chain elongation from co-fermented sludge and food waste. Journal of Cleaner Production, 292, 126009-126009.

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Monitoring Microbial Biomass Growth

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Optical density at 600 nm wavelength (OD 600 ) was used to monitor the increase in turbidity of pre-cultures, which is assumed to be correspondent to biomass growth only in balanced growth conditions, in a medium containing no hydrophobic compounds (Kalscheuer et al., 2007) (link).
Biomass concentration, as total suspended nitrogen (TSN), was determined using cuvette test LCK238, and the ammonium (NH 4 þ ) depletion was followed by cuvette test LCK 303 (Hach Lange ® , Germany).
COD, TN, nitrates, nitrites were estimated using the commercial kits LCK 914, LCK 338, LCK 340, LCK 341, LCK 303 (Hach-Lange ® , Germany). All analyses were done in triplicate, following the procedures recommended in kits protocol.

Da Silva P.D.M.P., Lima F., Alves M.M., Bijmans M.F.M, & Pereira M.A. (2016). Valorization of lubricant-based wastewater for bacterial neutral lipids production: Growth-linked biosynthesis. Water research, 101.

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Wastewater Parameter Determination

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Chemical oxygen demand (COD), total nitrogen (TN), ammonium (NH 4 þ ), phosphate (PO 4 3À
), nitrates, nitrites and total ammonia nitrogen present in the wastewater were determined using the cuvette-tests Lck914, Lck338, Lck386, Lck305, Lck340, Lck341 and Lck303, respectively (Hach-Lange, Germany). These measurements were made in triplicate, according to the manufacturer's procedures.

Castro A.R., Silva P.T.S., Castro P.J.G., Alves E., Domingues M.R.M, & Pereira M.A. (2018). Tuning culturing conditions towards the production of neutral lipids from lubricant-based wastewater in open mixed bacterial communities. Water research, 144.

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