Cd28 anti mouse fitc

Manufactured by BioLegend

CD28 anti-mouse FITC is a flow cytometry reagent that binds to the mouse CD28 cell surface receptor. It is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate) to allow for detection and analysis of CD28-positive cells.

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Lab products found in correlation

Pe anti mouse cd4, by BioLegend (2 mentions) Percp anti mouse cd8, by BioLegend (2 mentions) Facsmelody, by BD (2 mentions)

2 protocols using cd28 anti mouse fitc

Multicolor Flow Cytometry for Spleen CD4+/CD8+ T Cells

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The spleen samples were stained with CD4 anti-mouse PE (Biolegend), CD8 anti-mouse PerCP (Biolegend), and CD28 anti-mouse FITC (Biolegend). Samples were prepared according to the Biolegend protocol, and flow cytometry was performed with BD FACSMelody (BD Bioscience). The number of cells was analyzed by BD Cell Quest software. The analysis resulted in the percentage (%) of cells. Based on this, we measured the percentage of CD4

Handono K., Pratama M.Z., Sermoati I.A., Yuniati M.G., Haryati N.P.S., Norahmawati E., Endharti A.T., Irwanto Y., Solikhin M.B., & Hidayat S. (2021). The Effect of Mango Mistletoes (Dendrophthoe pentandra) Leaves Extract on Percentage of CD4+CD28+, CD8+CD28+, and interleukin-2 Levels of Aged Balb/c Mice. Open Access Macedonian Journal of Medical Sciences, 9(A), 414-421.

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Analysis of T-cell Subsets in BALB/c Mice

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Initially, splenocyte isolation from balb/c mouse spleen was performed aseptically after the mouse was sacrificed under deep ether anesthesia. Samples were prepared according to the Biolegend protocol. The spleen samples were colored with CD4 anti-mouse PE (Biolegend, Cat number 100408), CD8 anti-mouse PerCP (Biolegend, Cat number 140417), and CD28 anti-mouse FITC (Biolegend, Cat number 122008). The flow cytometry was carried out with BD FACSMelody by the manufacturer's instructions. The percentages of CD8 +

Handono K., Sunarti S., Pratama M.Z., Hidayat S., Solikhin M.B., Sermoati I.A., & Yuniati M.G. (2022). The Mango’s Mistletoe Leaves Extract Ameliorates Lupus by Inhibiting the Anti-dsDNA Antibody Production, the Percentages of CD8+CD28− and CD4+CD28− T Cells. Open Access Macedonian Journal of Medical Sciences, 10(A), 248-255.

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