LUVs, mimicking the membrane domain involved in epithelial cell–ECM adhesion (Márquez et al. 2008 (link)), were prepared in pH 7.4 buffer containing 10.0 mM HEPES, 50.0 mM KCl, 1.0 mM EDTA, and 3.0 mM NaN3, as previously described (Su et al. 2019). The lipid composition was with a lipid molar ratio of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC): 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE): 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS): 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoinositol(POPI) : sphingomyelin (SM) = 40:25:15:10:10 (Avanti Polar Lipids). Note that POPC, POPE, and SM have neutral charge, while POPS and POPI are negatively charged. Therefore, the ECM-adhesion-imitating LUVs used here are about 25% anionic. The size of the LUVs was 400 nm.
1 palmitoyl 2 oleoyl sn glycero 3 phosphoinositol
Manufactured by Avanti Polar Lipids
Sourced in United States
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoinositol is a synthetic phospholipid with a palmitic acid chain at the sn-1 position and an oleic acid chain at the sn-2 position. It contains a phosphoinositol headgroup.
Automatically generated - may contain errors
Lab products found in correlation
1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (4 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (2 mentions)
1 palmitoyl 2 oleoyl glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine n lissamine rhodamine b sulfonyl rh pe, by Avanti Polar Lipids (1 mentions)
1 palmitoyl 2 oleoyl phosphatidylglycerol, by Avanti Polar Lipids (1 mentions)
1 palmitoyl 2 oleoylphosphatidylcholine, by Avanti Polar Lipids (1 mentions)
Cholesterol chol, by Merck Group (1 mentions)
1 2 dimyristoyl sn glycerol, by Cayman Chemical (1 mentions)
N stearoyl d erythro sphingosine, by Cayman Chemical (1 mentions)
1 2 dioleoyl sn glycero 3 phosphate, by Avanti Polar Lipids (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphate, by Avanti Polar Lipids (1 mentions)
1 stearoyl 2 arachidonoyl sn glycero 3 phosphate, by Avanti Polar Lipids (1 mentions)
1 stearoyl 2 docosahexaenoyl sn glycero 3 phosphate, by Avanti Polar Lipids (1 mentions)
1 stearoyl 2 arachidonoyl sn glycero 3 phospho, by Avanti Polar Lipids (1 mentions)
Perchloric acid, by Avantor (1 mentions)
Milli q water, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Ammonium formate, by Merck Group (1 mentions)
6 protocols using 1 palmitoyl 2 oleoyl sn glycero 3 phosphoinositol
1
Preparation of ECM-Mimicking Lipid Vesicles
2
Lipid Procurement for Biological Studies
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Except from the extracted mitochondrial lipids used in this work, we purchased lipids 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (PC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (PE), bovine brain sphingomyelin (SM) and bovine heart cardiolipin (CL), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoinositol (PI), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (PS), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (RhPE) and cholesterol (Chol) from Avanti Polar Lipids (Alabaster, AL, United States).
3
Lipid Protocols for Membrane Research
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Lipids were purchased from Avanti Polar Lipids: 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), 1-palmitoyl-2-oleoylphosphatidylglycerol (POPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoinositol (POPI, PI), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′-phosphate) [PI(4)P], 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,4′-bisphosphate) [PI(3,4)P2], 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′-bisphosphate) [PI(4,5)P2], 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,4′,5′-trisphosphate) [PI(3,4,5)P3]. All other reagents and chemicals, such as for recording solutions and cell culture media, were purchased from FUJIFILM Wako Pure Chemical or Nacalai Tesque.
4
Liposome Preparation and Composition
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Large unilamellar vesicles (LUVs), with a diameter of approximately 100 nm, were obtained by extrusion of multilamellar vesicles (MLVs) as described elsewhere [41 (link)]. 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoinositol (POPI), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) were from Avanti Polar Lipids (Alabaster, AL, USA), while cholesterol (Chol) and ergosterol (Erg) were from Sigma. The LUVs studied were mostly zwitterionic (pure POPC, POPC:Chol 70:30, POPC:Erg 70:30, and C. albicans membrane-like mixture, i.e., POPC:POPE:POPS:POPI:Erg 59:21:3:4:13 [42 (link)]), mimicking membrane systems relevant for the study [32 (link),41 (link)]. Liposomes were extruded in the day of the measurement. HEPES buffer was used in all the measurements, except when otherwise indicated.
5
Lipids and Detergents for Structural Studies
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Lipids: 1,2-dipalmitoyl-sn-glycero-3-phosphate (16:0/16:0-PA), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoinositol (16:0/18:1-PI), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (16:0/18:1-PS), 1,2-dioleoyl-sn-glycero-3-phosphate (18:1/18:1-PA), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoinositol (18:0/20:4-PI), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phospho-(1'-myo-inositol-4',5'-bisphosphate) (18:0/20:4-PI(4,5)P2), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphate (18:0/20:4-PA), and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphate (18:0/22:6-PA) were obtained from Avanti Polar Lipids. 1,2-dimyristoyl-sn-glycerol (14:0/14:0-DG), 1-palmitoyl-2-oleoyl-sn-3-phosphoglycerol (16:0/18:1-PG), and N-stearoyl-D-erythro-sphingosine (d18:1/18:0-Cer) were obtained from Cayman Chemical Company. 16:0/18:1-sn-glycero-3-phosphocholine (16:0/18:1-PC) was purchased from Sigma-Aldrich.
Detergents: n-dodecyl-β-D-maltoside (DDM) was obtained from Cayman Chemical Company. Cholesteryl hemisuccinate (CHS) was purchased from Sigma-Aldrich.
Detergents: n-dodecyl-β-D-maltoside (DDM) was obtained from Cayman Chemical Company. Cholesteryl hemisuccinate (CHS) was purchased from Sigma-Aldrich.
6
Phospholipid Analysis Protocol
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The phospholipid
standard 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoinositol
(POPI, PI(16:0/18:1)), the lipid mixturel -α-phosphatidylinositol,
and the 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine
(dMPE; PE 14:0/14:0) were purchased from Avanti Polar Lipids, Inc.
(Alabaster, AL, USA) and used without further purification. Milli-Q
water (Milli-Q H2O) was used for all the experiments, filtered
through a 0.22 μm filter, and obtained with Milli-Q Millipore
system (Synergy, Millipore Corporation, Billerica, MA, USA). HPLC
grade acetonitrile, methanol (MeOH), and chloroform (CHCl3) were obtained from Fisher Scientific Ltd. (Leicesterscire, UK).
Ammonium formate, formic acid, and solid nitronium tetrafluoroborate
(NO2BF4) were obtained from Sigma-Aldrich (St.
Louis, MO, USA). Sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O) and ammonium molybdate were
purchased from Piedel-de-Haën (Seelze, Germany). Ascorbic acid
and perchloric acid 70% were purchased from VWR International (Leicestershire,
UK) and from Panreac (Barcelona, Spain), respectively.
standard 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoinositol
(POPI, PI(16:0/18:1)), the lipid mixture
and the 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine
(dMPE; PE 14:0/14:0) were purchased from Avanti Polar Lipids, Inc.
(Alabaster, AL, USA) and used without further purification. Milli-Q
water (Milli-Q H2O) was used for all the experiments, filtered
through a 0.22 μm filter, and obtained with Milli-Q Millipore
system (Synergy, Millipore Corporation, Billerica, MA, USA). HPLC
grade acetonitrile, methanol (MeOH), and chloroform (CHCl3) were obtained from Fisher Scientific Ltd. (Leicesterscire, UK).
Ammonium formate, formic acid, and solid nitronium tetrafluoroborate
(NO2BF4) were obtained from Sigma-Aldrich (St.
Louis, MO, USA). Sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O) and ammonium molybdate were
purchased from Piedel-de-Haën (Seelze, Germany). Ascorbic acid
and perchloric acid 70% were purchased from VWR International (Leicestershire,
UK) and from Panreac (Barcelona, Spain), respectively.
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