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2 protocols using pc 16 0 16 0

1

Lipid Profiling Using HPLC-MS

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All reagents were purchased from Sigma-Aldrich (Saint Louis, MO, USA) unless specified. The liquid chromatography solvent was HPLC-grade and purchased from Acros Organics. Synthetic lipid standards (Cer d18:1/18:0, Cer d18:1/15:0, PE 12:0/12:0, PE 16:0/16:0, PC 13:0/13:0, PC 16:0/16:0, SM d18:1/18:0, SM d18:1/12:0, stigmasterol, cholesteryl heptadecanoate and glyceryl trinonadecanoate) were purchased from Avanti Polar Lipids and Sigma-Aldrich.
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2

Lipidomic Analysis of Synthetic Standards

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The liquid chromatography solvent, acetonitrile, was HPLC-grade and purchased from Acros Organics. Ammonium formate (>99%) was supplied by Sigma Aldrich. Synthetic lipid standards (Cer d18:1/18:0, Cer d18:1/15:0, PE 12:0/12:0, PE 16:0/16:0, PC 13:0/13:0, PC 16:0/16:0, SM d18:1/18:0, SM d18:1/12:0) were purchased from Avanti Polar Lipids.
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