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Kx 21n analyzer

Manufactured by Sysmex
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Sourced in Japan

The KX-21N is a compact, automated hematology analyzer that provides accurate and reliable analysis of complete blood count (CBC) parameters. It is designed to deliver efficient and cost-effective blood cell analysis in a variety of clinical settings.

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Lab products found in correlation

Doxycycline, by Merck Group (1 mentions)

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KX-21N (207 citations) KX-21N hematology analyzer (17 citations) SYSMEX Automated Hematology Analyzer (KX-21N, (2 citations) KX-21N haematology analyzer (2 citations)

6 protocols using kx 21n analyzer

1

Fasting Blood Sampling for Hematological Analysis

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Fasting (10-12 h) blood samples were drawn from the antecubital vein in the morning. Only water was allowed to be consumed ad libitum before the samples were drawn. They were collected into two vacuum tubes 5/2 ml K2E (B&D Vacutainer, Plymouth, UK), one 5/3 ml serum gel tube (B&D Vacutainer, Plymouth, UK) and one 5/2 ml FX-tube (B&D Vacutainer, Plymouth, UK). Haematological analyses were done immediately after blood draws with a Sysmex KX 21N-analyzer (Sysmex Co., Kobe, Japan). Serum
Kyröläinen H., Hackney A.C., Salminen R., Repola J., Häkkinen K, & Haimi J. (2018). Effects of Combined Strength and Endurance Training on Physical Performance and Biomarkers of Healthy Young Women. Journal of strength and conditioning research, 32(6).
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2

Murine Spleen Cell Isolation

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Spleens from 4-month-old mice were harvested and manually dissociated into single cell suspensions in FACS buffer (PBS containing 10% FBS and 0.2 mM EDTA). After erythrocyte lysis with ACK (ammonium-chloride-potassium) lysing buffer for 2 min at room temperature (R/T), total cellularity was determined by direct counting using a Sysmex KX-21 N analyzer (Sysmex).
Bachiller S., Hidalgo I., Garcia M.G., Boza-Serrano A., Paulus A., Denis Q., Haikal C., Manouchehrian O., Klementieva O., Li J.Y., Pronk C.J., Gouras G.K, & Deierborg T. (2022). Early-life stress elicits peripheral and brain immune activation differently in wild type and 5xFAD mice in a sex-specific manner. Journal of Neuroinflammation, 19, 151.
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3

Fasting and Blood Analysis Protocol

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Rats were fasted overnight before blood collection as well as 24 h after SDNP exposure. Blood collection was carried out for all rats by heart puncture. Hematological tests were performed using a KX-21N analyzer (Sysmex, Kobe, Japan). An additional portion from each blood sample was centrifuged at 3,000 rpm for 5 min at room temperature for serum separation. Serum samples were assayed immediately to determine selected biochemical parameters using a BS-300 Auto Chemistry Analyzer (Golden Harvest Industries, Chennai, India).
Almansour M., Alarifi S, & Jarrar B. (2018). In vivo investigation on the chronic hepatotoxicity induced by intraperitoneal administration of 10-nm silicon dioxide nanoparticles. International Journal of Nanomedicine, 13, 2685-2696.
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4

Blood Biomarker Assessment Protocol

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Blood samples were drawn from the antecubital vein in a sitting position. Analysis included hemoglobin (Hb), serum total testosterone, cortisol, growth hormone, lactate, and pH. Serum samples were kept frozen at −80°C until analyzed. Two milliliters of blood were taken in K2 EDTA tubes (Terumo Medical Co., Leuven, Belgium) for measurements of Hb concentration with a Sysmex KX 21N Analyzer (Sysmex Co., Kobe, Japan). For the determination of serum hormone concentrations, five milliliters of blood were taken into serum separator tubes and the concentrations were analyzed by an immunometric chemiluminescence method with Immulite® 1000 (DPC, Los Angeles, USA). The sensitivities of the assays were 0.5 nmol/l for testosterone, 5.5 nmol/l for cortisol, and 2.6 µg/l for growth hormone. The intra-assay coefficient of variation (CV) was 5.7% for testosterone, 4.6% for cortisol, and 4.2% for growth hormone. Blood samples for lactate were obtained from the fingertip and collected into capillary tubes (20 µl), which were placed in a 1 ml hemolyzing solution and analysed automatically after the completion of testing according to the manufacturer’s instructions (EKF diagnostic, C-line system, Biosen, Germany). pH was analyzed with IL GEM Premier 3000 Blood Gas System (Instrumentation Laboratory, Lexington, MA, USA). The intra-assay CV was 0.1% for pH.
Mero A., Tornberg J., Mäntykoski M, & Puurtinen R. (2015). Effects of far-infrared sauna bathing on recovery from strength and endurance training sessions in men. SpringerPlus, 4, 321.
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5

Hematological Analyzer Sysmex KX-21N

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The Sysmex KX-21N analyzer was used to determine the following hematological parameters: white blood cells and platelets.
Bardaa S., Turki M., Ben Khedir S., Mzid M., Rebai T., Ayadi F, & Sahnoun Z. (2020). The Effect of Prickly Pear, Pumpkin, and Linseed Oils on Biological Mediators of Acute Inflammation and Oxidative Stress Markers. BioMed Research International, 2020, 5643465.
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6

Studying Rps19 Deficiency in Mice

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All animal work was approved by the local Swedish ethical committee for experimental animal research. Rps19-deficient mice [16 (link)] and HRI −/− mice [21 (link)] were crossed for at least 5 generations, here referred to as Rps19-HRI mice. Rps19-deficiency is induced by Doxycycline administration that together with a constitutively active trans-activator rtTA drives the expression of a shRNA against Rps19. For all experiments mice homozygote for the rtTA trans-activator was used. All mice had either wild type expression of HRI (HRI+/+) or were heterozygote (HRI+/−). 7–9 weeks old wild type C57/BL6 females were irradiated (900cGy) and transplanted with 2 million total bone marrow cells from mice either WT or heterozygote for Rps19 shRNA (via tail vein injection). Mice received Ciprofloxacin 125mg/ml (Arrow Generics, Kilmersdon, UK) 14 days post transplantation. After 8–11 weeks of engraftment Rps19-deficiency was induced for 10–11 days by 0.5mg/ml Doxycycline (Sigma-Aldrich, Saint Louis, US) and 10mg/ml sucrose in drinking water. Blood samples were collected from tail vein 7–14 days before and 9–10 days after Doxycycline administration and analyzed on KX-21N analyzer (Sysmex, Kobe, Japan).
Wuyts J., Van de Peer Y., Winkelmans T, & De Wachter R. (2002). The European database on small subunit ribosomal RNA. Nucleic Acids Research, 30(1), 183-185.
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