Fluo 4am s1060

GAS (SMB00313), CoCl₂ (232696), and calcium ionophore (A23187) were purchased from Sigma-Aldrich (St Louis, MO, USA). GAS and CoCl₂ were dissolved in phosphate-buffered solution to prepare the original solution of 100 mM, which was stored at -20° C. CQ diphosphate (HY-17589) and 3-BDO (HY-U00434) were purchased from MedChemExpress. Fluo-4AM (S1060) was obtained from Beyotime Biotechnology, Inc. (Nanjing, China). Primary antibodies against β-actin (66009-1-Ig, 1:10 000), LC3 (14600-1-AP, 1:1000), p62 (18420-1-AP, 1:1000), p62(66184-1-lg), LAMP-2 (66301-1-lg, 1:1000), and CaMKIIα (11533-1-AP, 66843-1-Ig) were purchased from Proteintech (Wuhan, China). Rabbit anti-p-p62 (Thr349) (Ab211324) was purchased from Abcam (Cambridge, MA, USA). Primary antibodies against CaMKIIα (#4436, 1:1000) and anti-p-CaMKIIα (Thr286) (#12716, 1:1000) were obtained from Cell Signaling Technology (Danvers, MA, USA). Goat anti-rabbit IgG (H&L)-HRP (BS13278, 1:10000) and goat anti-mouse IgG (H&L)-HRP (BS12478) were obtained from Bioworld. Goat PAb to Rb IgG Alexa Fluor®488 (Ab150077, 1:200) and goat PAb to MS IgG Alexa Fluor®647 (Ab150115) were purchased from Abcam. A fluorescein isothiocyanate-conjugated goat anti-rabbit secondary antibody (A0516, 1:200) was purchased from Beyotime Biotechnology.

FITC-conjugated anti-human CD62P antibody (304904) was purchased from Biolegend (San Diego, CA, USA). FITC-conjugated PAC-1 (340507) was purchased from BD Biosciences (San Jose, CA, USA). JC-1 (C2005) and Fluo-4 AM (S1060) were purchased from the Beyotime Institute of Biotechnology (Beyotime, Shanghai, China). FITC-conjugated lactadherin (BLAC-FITC) was purchased from Haematologic Technologies (Essex Junction, VT, USA). ADP (384), Collagen (385), Thrombin (386), ATP Standard (387), and Luciferin/luciferase reagents (395) were purchased from Chrono-log Corporation (Havertown, PA, USA). U46619 (538944) was purchased from Calbiochem (La Jolla, CA, USA). PAGE Gel Fast Preparation Kit (PG 113) was purchased from Yazin Biotechnology (Shanghai, China). Antibodies for western blot against β-Actin (4970), GAPDH (2118), caspase-3 (9662), Bcl-xL (2764), Bak (D2D3) (6947), P53 (7F5) (2527), phospho-P53 (Ser46) (2521), phospho-VASP (Ser157) (3111), PKC α (2056), PKC δ (2058), Akt (9272), and phospho-Akt (Thr308) (13038) were purchased from Cell Signaling Technology (Beverly, MA, USA).