HepG2 hepatoma cells were obtained from the American Type Culture Collection (ATCC). The cells were cultured in Dulbecco’s modified Eagle medium (DMEM; WelGENE) supplemented with 10% fetal bovine serum (FBS), 1 U/ml penicillin, and 1 μg/ml streptomycin and incubated at 37°C in a 5% CO2 atmosphere.
Hepg2 hepatoma cells
Manufactured by American Type Culture Collection
Sourced in United States
HepG2 hepatoma cells are a well-established human liver cancer cell line. They are derived from a liver hepatocellular carcinoma and are commonly used in research to study liver-related processes and diseases.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
Dulbecco s minimum essential medium, by Thermo Fisher Scientific (1 mentions)
Chang liver cells, by American Type Culture Collection (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic solution aa, by Thermo Fisher Scientific (1 mentions)
Mouse c2c12 myoblast, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
Sh sy5y, by Thermo Fisher Scientific (1 mentions)
Sh sy5y neuroblastoma cells, by American Type Culture Collection (1 mentions)
Crl 2061, by Thermo Fisher Scientific (1 mentions)
Hela cervical carcinoma cells, by American Type Culture Collection (1 mentions)
4 protocols using hepg2 hepatoma cells
1
HepG2 Cell Culture Protocol
2
Culturing Liver Cell Lines
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Chang liver cells, PLC/PRF/5 hepatoma cells, and HepG2 hepatoma cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). Dulbecco's minimum essential medium (Gibco, USA) supplemented with 10% fetal bovine serum, 50 unit/ml penicillin, non-essential amino acids and HEPES was used for culture at 37°C in presence of 5% CO2. Medium was replaced and confluent growth was split regularly.
3
Oleic acid treatment of HepG2 cells
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HepG2 hepatoma cells were obtained from the American Type Culture Collection. The HepG2 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Billings, MT, USA) supplemented with 10% fetal bovine serum (FBS) at 37 °C with 5% CO2 and were allowed to reach 70–80% confluency. The cells were then treated with 0.6 mM oleic acid (#01257; Sigma, St. Louis, MO, USA) with or without RBE, then harvested for downstream molecular and imaging analysis.
4
Cell Culture of Various Human and Rodent Cell Lines
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Human myoblasts generated by the immortalization of primary cultured human myogenic cells were a kind gift from Dr. Hashimoto (Hashimoto et al., 2006 (link)). CRL-2061 and CCL-136 rhabdomyosarcoma cells, HeLa cervical carcinoma cells, SH-SY5Y neuroblastoma cells, HepG2 hepatoma cells, AGS gastric adenocarcinoma and HEK human embryonal kidney cells, and rat L6 cells and mouse C2C12 myoblasts were obtained from the American Type Culture Collection (ATCC; Manassas, VA, United States). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco Life Technologies, Waltham, MA, United States), except for CRL-2061 that were cultured in RPMI (Gibco Life Technologies, Waltham, MA, United States) and SH-SY5Y and HepG2 that were cultured in Minimum Essential Medium (MEM) (Gibco by Life Technologies, Grand Island, NY, United States). Media were supplemented with 10% fetal bovine serum (FBS; Gibco Life Technologies) and 1% antibiotic-antimycotic solution (AA; Gibco Life Technologies) at 37°C in a 5% CO2 humidified incubator.
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