A PA-labelled sugar chain was generated using a BlotGlyco kit (Sumitomo Bakelite Co., Ltd) according to the manufacturer's instructions. The culture supernatant was separated using a Superdex peptide column and eluted with distilled water at a flow rate of 1 ml min−1. The eluent was monitored by ultraviolet spectrophotometry at 400 nm.
Blotglyco kit
Manufactured by Sumitomo Bakelite
Sourced in Japan
The BlotGlyco kit is a laboratory product manufactured by Sumitomo Bakelite. It is designed for the analysis of glycans in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Pngase f, by New England Biolabs (1 mentions)
Sepharose cl 4b, by Merck Group (1 mentions)
Lc ms grade ammonium formate, by Merck Group (1 mentions)
Rapid pngase f, by New England Biolabs (1 mentions)
Bovine fetuin, by Merck Group (1 mentions)
Pa glucose oligomer, by Takara Bio (1 mentions)
Ribonuclease b rnase b, by Merck Group (1 mentions)
3 protocols using blotglyco kit
1
Affinity Purification of PA-Labeled Glycans
2
Glycan Release and Fluorescent Labeling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
N‐glycans were released from EVs and whole cell lysates by treatment with peptide‐N4‐(N‐acetyl‐β‐glucosaminyl)asparagine amidase F (PNGase F) (New England Biolabs, Ipswich, MA, USA) according to the manufacturer's instructions. The released N‐glycans were purified and fluorescently labeled with 2‐aminopyridine (PA) using a BlotGlyco Kit (Sumitomo Bakelite, Tokyo, Japan) according to the manufacturer's instructions.
3
Comprehensive Glycoprotein Analysis Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bevacizumab (Avastin) and
trastuzumab (Herceptin)
were purchased from Chugai Pharmaceutical (Tokyo, Japan). Nivolumab
(Opdivo) was purchased from Ono Pharmaceutical (Osaka, Japan), and
ramucirumab (Cyramza) was purchased from Eli Lilly Japan (Hyogo, Japan).
Rituximab (Rituxan) was obtained from Zenyaku Kogyo (Tokyo, Japan).
Rapid PNGase F was purchased from New England Biolabs (Tokyo, Japan).
The BlotGlyco kit was from Sumitomo Bakelite Co. Ltd. (Tokyo, Japan),
and PA-glucose oligomer was obtained from TaKaRa (Kyoto, Japan). Sepharose
CL-4B, ribonuclease B (RNase B), bovine fetuin, and LC/MS-grade ammonium
formate were obtained from Sigma-Aldrich (St. Louis, MO). All other
unspecified reagents were products of FUJIFILM Wako Pure Chemical
Corporation (Osaka, Japan).
trastuzumab (Herceptin)
were purchased from Chugai Pharmaceutical (Tokyo, Japan). Nivolumab
(Opdivo) was purchased from Ono Pharmaceutical (Osaka, Japan), and
ramucirumab (Cyramza) was purchased from Eli Lilly Japan (Hyogo, Japan).
Rituximab (Rituxan) was obtained from Zenyaku Kogyo (Tokyo, Japan).
Rapid PNGase F was purchased from New England Biolabs (Tokyo, Japan).
The BlotGlyco kit was from Sumitomo Bakelite Co. Ltd. (Tokyo, Japan),
and PA-glucose oligomer was obtained from TaKaRa (Kyoto, Japan). Sepharose
CL-4B, ribonuclease B (RNase B), bovine fetuin, and LC/MS-grade ammonium
formate were obtained from Sigma-Aldrich (St. Louis, MO). All other
unspecified reagents were products of FUJIFILM Wako Pure Chemical
Corporation (Osaka, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!