For SEM, samples were dehydrated by sequential immersion in ascending concentrations of ethanol (30, 50, 75, 90 and 100% (v/v) in water) for 5 min per concentration, followed by critical point drying in hexamethyldisilazane for 10 min. Samples were subsequently transferred onto carbon tape and attached to SEM sample holders. A Quorum SC7620 Mini Sputter Coater Glow Discharge System was used for gold–palladium coating (for 15 s) of fiber samples, in order to enable the use of the equipment in high vacuum mode. Fiber characterization and cell attachment images were obtained using a Carl Zeiss Evo LS15 VP-scanning electron microscope.
Sc7620 mini sputter coater glow discharge system
Manufactured by Quorum Technologies
Sourced in United Kingdom
The SC7620 Mini Sputter Coater/Glow Discharge System is a compact, versatile equipment designed for the deposition of thin films onto various substrates. It can perform both sputtering and glow discharge functionalities within a single unit. The system is suitable for a range of applications that require thin film coatings.
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Lab products found in correlation
9 protocols using sc7620 mini sputter coater glow discharge system
1
SEM Fiber Characterization and Cell Attachment
2
SEM analysis of 3D material structure
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The examination of structure and cross-section of 3D materials was carried out by scanning electron microscopy (SEM) imaging (Quanta 3D FEG scanning electron microscope, Quorum Technologies, Lewes, UK). A thin layer of gold and palladium (SC7620 Mini Sputter Coater/Glow Discharge System, Quorum Technologies, Lewels, UK) was applied on the surface of the samples before the analysis. The diameters of PLA microspheres were measured by SEM after incorporation into materials.
3
SEM Analysis of Crosslinked Microspheres
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Scanning electron microscopy (SEM) imaging was performed using the Quanta 3D FEG scanning electron microscope manufactured by Quorum Technologies (Lewes, UK). The matrices and crosslinked microspheres were subjected to SEM analysis to examine the surface structure of the obtained materials. Prior to the analysis, a thin layer of gold and palladium was sprayed onto the surface of the samples (SC7620 Mini Sputter Coater/Glow Discharge System, Quorum Technologies).
4
Bacterial Cell Morphology via SEM
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Outer morphology of the bacterial cells before and after biosorption was examined using SEM (Carl Zeiss Supra 55 Gemin; German Technology, Jena, Germany). Prepared samples were placed on the sample holder (stub) with carbon tape. In order to increase the electron conduction and to improve the quality of micrographs, a conductive layer of gold was made with portable SC7620 ‘Mini’ sputter coater/glow discharge system (Quorum Technologies Ltd, Laughton, UK) (Michalak et al. 2014 ).
5
Scanning Electron Microscopy Protocol
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SEM analyses performed using a Thermo Scientific microscope (Waltham, MA, USA) model Prisma E, from the SENAI CETIQT Characterization Laboratory, equipped with an energy-dispersive X-ray spectrometer (EDS). The equipment was operated with an electron acceleration voltage of 20 kV in backscattering and secondary electrons. Preparation samples by gold deposition (sputtering) using an SC7620 Mini Sputter Coater/Glow Discharge System by Quorum. A vacuum with an ultra-pure argon atmosphere was used, applying a current of 15 mA for 60 s and a gold target.
6
Visualization of Therapeutic Monoclonal Antibodies
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4 µl of therapeutic mAbs, at a concentration of 400 μg/ml, was placed on the carbon face of 200 mesh glow discharged carbon coated copper grids (Agar Scientific) and allowed to adsorb for 1 min. Grids were glow discharged for 60 s at 20 mA (SC7620 Mini Sputter Coater/Glow Discharge system, Quorum Technologies), prior to usage. Grids were washed 3 times with 10 µl of double distilled water, followed by blotting with Whatmann filter paper. For staining, 4 µl of 2% uranyl acetate, pH ~ 7.0 was applied onto the grid twice, for a time period of 20 s and 40 s, respectively, and the surplus stain was removed by blotting in each case. The grid was dried at room temperature for 2 min and visualized at a magnification of 100,000x in a FEG-TEM (FEI Tecnai) operating at 200 kV. Three micrographs from different grid squares were collected for each sample.
7
SEM Imaging of Cells with Critical Point Drying
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For scanning electron microscope (SEM) imaging, cell samples were dehydrated by sequential immersion in graded ethanol (30%, 50%, 75%, 90% and 100%) for 5min per concentration, followed by critical point drying in Hexamethyldisilazane (HDMS) for 10min. Samples are then transferred onto carbon tape and attached to SEM sample holders. A Quorum SC7620 Mini Sputter Coater Glow Discharge System was used for gold-palladium coating (15s) of fiber samples, in order to enable the use of the equipment in High Vacuum mode. Fiber characterization and cell attachment images were obtained using a Carl Zeiss Evo LS15 VP-Scanning Electron Microscope.
8
Alginate Bead Morphological Characterization
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The morphological characterization of the different alginate beads was conducted using a Phenom-World desktop scanning electron microscope (SEM) (Phenom-World, Eindhoven, The Netherlands) with a tungsten filament operating at 10 kV and a charge reduction sample holder. The samples were observed after sputter coating on a Mini Sputter Coater/Glow Discharge System SC7620 (Quorum Technologies Ltd., Lewes, UK).
9
Microstructural Analysis of Lyophilized UG Scaffolds
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The microstructure of the lyophilized UG scaffolds was investigated using a PhenomWorld desktop scanning electron microscope (SEM) (Phenom-World, Eindhoven, The Netherlands) with a tungsten filament (10 kV) and a charge reduction sample holder. The samples were inspected after sputter coating on a Mini Sputter Coater/Glow Discharge System SC7620 (Quorum Technologies Ltd, East Sussex, UK).
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