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Anti cd4 and anti cd8 macs beads

Manufactured by Miltenyi Biotec
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Sourced in United States

Anti-CD4 and anti-CD8 MACS beads are magnetic beads coated with antibodies specific to the CD4 and CD8 surface markers, respectively. These beads are used for the isolation and purification of CD4+ and CD8+ T cells from cell samples.

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Lab products found in correlation

Facsaria, by BD (3 mentions) Anti cd4 macs beads, by BD (2 mentions) Tcrγδ, by BD (1 mentions)

Close spelling variants of this product

CD4 and CD8 MACS beads CD8 MACS beads CD4+ MACS beads Anti-CD8 beads Anti-CD4 beads MACS beads Anti-CD8 Anti-CD4

3 protocols using anti cd4 and anti cd8 macs beads

1

Purification and Transfer of T Cell Subsets

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CD45.1+ CD4+ and CD45.1+ CD8+ T cells were purified from the spleens of naïve B6.CD45.1+ mice by positive selection with anti-CD4 and anti-CD8 MACS beads (Miltenyi Biotec). CD4+Foxp3+CD25hi, CD4+Foxp3+CD25lo and CD4+Foxp3− T cells were sorted from spleens of Foxp3-DTR/GFP mice using a FACSAria (BD Biosciences) after positive selection with anti-CD4 MACS beads. Sorted cells (5 × 105) were transferred intravenously into naïve Tcrb−/−Tcrd−/− recipient mice. All sorted T-cell subsets were more than 95% pure.
Botta D., Fuller M.J., Marquez-Lago T.T., Bachus H., Bradley J.E., Weinmann A.S., Zajac A.J., Randall T.D., Lund F.E., León B, & Ballesteros-Tato A. (2017). Dynamic regulation of T Follicular Regulatory cell responses by interleukin 2 during influenza infection. Nature immunology, 18(11), 1249-1260.
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2

Purification and Transfer of T Cell Subsets

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CD45.1+ CD4+ and CD45.1+ CD8+ T cells were purified from the spleens of naïve B6.CD45.1+ mice by positive selection with anti-CD4 and anti-CD8 MACS beads (Miltenyi Biotec). CD4+Foxp3+CD25hi, CD4+Foxp3+CD25lo and CD4+Foxp3− T cells were sorted from spleens of Foxp3-DTR/GFP mice using a FACSAria (BD Biosciences) after positive selection with anti-CD4 MACS beads. Sorted cells (5 × 105) were transferred intravenously into naïve Tcrb−/−Tcrd−/− recipient mice. All sorted T-cell subsets were more than 95% pure.
Botta D., Fuller M.J., Marquez-Lago T.T., Bachus H., Bradley J.E., Weinmann A.S., Zajac A.J., Randall T.D., Lund F.E., León B, & Ballesteros-Tato A. (2017). Dynamic regulation of T Follicular Regulatory cell responses by interleukin 2 during influenza infection. Nature immunology, 18(11), 1249-1260.
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3

Isolation and Purification of DN Thymocyte Subsets

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CD4–CD8– DN thymocytes were negatively selected with anti-CD4 and anti-CD8 MACS beads according to the protocol of the manufacturer (Miltenyi Biotec, Auburn, CA, United States). After staining with antibodies against lineage (Lin) markers B220, CD19, CD11b, Gr1, CD11c, NK1.1, Ter119, CD3ε, CD8α, CD8β, TCRβ, and TCRγδ (BD Pharmingen), DN2 thymocytes (c-KithiCD25hiLin–) were purified based on staining with anti-c-Kit and anti-CD25 antibodies (BD Pharmingen) by cell sorting on FACSAria (BD Biosciences) and analyzed on the LSR-II (BD Biosciences). DN3a (c-Kit–/loCD25hiLin–CD27lo) cells were purified by sorting after staining with anti-CD27 antibody (eBioscience, San Diego, CA, United States).
Dong Y., Guo H., Wang D., Tu R., Qing G, & Liu H. (2021). Genome-Wide Analysis Identifies Rag1 and Rag2 as Novel Notch1 Transcriptional Targets in Thymocytes. Frontiers in Cell and Developmental Biology, 9, 703338.
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