Rabbit anti rnaseh2a

Manufactured by OriGene

Rabbit anti-RNASEH2A is an antibody that specifically targets the RNASEH2A protein. RNASEH2A is a subunit of the RNase H2 enzyme complex, which is involved in the removal of RNA primers during DNA replication and repair. This antibody can be used for the detection and analysis of RNASEH2A in various research applications.

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2 protocols using rabbit anti rnaseh2a

Antibody Characterization for Protein Detection

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The following antibodies were used for immunofluorescence (IF) and immunoblotting (IB) at indicated dilutions: sheep anti-pan-RNase H2 (raised against human recombinant RNase H213 (link), IB 1:1,000, IP 5 µl / 1 ml lysate); rabbit anti-RNASEH2C (Proteintech 16518-1-AP; IB 1:1,000); rabbit anti-RNASEH2A (Origene TA306706, IB 1:1,000); mouse anti-RNASEH2A (Abcam ab92876; IF 1:500); mouse anti-RNASEH2A G-10 (Santa Cruz Biotechnologies sc-515475; WB 1:1000); mouse anti-γH2AX JBW301 (Millipore 05-636, IF 1:800 – 1:2,000); rabbit anti-RAD51 H-92 (Santa Cruz Biotechnologies sc-8349, IF 1:150); rabbit anti-BRCA146 (link) (IB 1:1,000); mouse anti-Cas9 (Diagenode C15200203, IB 1:1,000); rabbit anti-PARP1 H-250 (Santa Cruz Biotechnologies sc-7150, IB 1:1,000); mouse anti-PAR 10H (Enzo ALX-804-220-R100, IB 1:1,000); rabbit anti-Topoisomerase I (Abcam ab109374; IB 1:5,000); rabbit anti-DYKDDDDK (Cell Signaling Technologies 2368, IB 1:1,000); rabbit anti-actin (Sigma A2066, IB 1:5,000); mouse anti-α–tubulin DM1A (Millipore CP06, IB 1:5,000); mouse anti-α–tubulin B512 (Sigma T6074, IB 1:5,000); rabbit anti-GAPDH (Sigma G9545, IB 1:20,000); mouse anti-vinculin (Sigma V9264, IB 1:1,000); rabbit anti-DNA polymerase beta (Abcam ab26343, IB 1:1,000); rabbit anti-cleaved caspase-3 (Cell Signaling Technologies 9661S, IF 1:800).

Zimmermann M., Murina O., Reijns M.A., Agathanggelou A., Challis R., Tarnauskaitė Ž., Muir M., Fluteau A., Aregger M., McEwan A., Yuan W., Clarke M., Lambros M.B., Paneesha S., Moss P., Chandrashekhar M., Angers S., Moffat J., Brunton V.G., Hart T., de Bono J., Stankovic T., Jackson A.P, & Durocher D. (2018). CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature, 559(7713), 285-289.

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Antibody Characterization for Protein Detection

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