For immunofluorescence, goat anti-rabbit IgG Alexa Fluor 633–conjugated (A21070) and goat anti-mouse IgG Alexa Fluor 633–conjugated (A21050) antibodies were from Thermo Fisher Scientific. Goat anti-rabbit IgG FITC (fluorescein)-conjugated (111–096-047), goat anti-mouse IgG FITC (fluorescein)-conjugated (115–096-072), goat anti-rabbit IgG Rhodamine (TRITC)–conjugated (111–025-046) and sheep anti-mouse IgG Rhodamine (TRITC)–conjugated (515–025-072) antibodies were from Jackson ImmunoResearch. For Western blotting, horse anti-mouse IgG HRP-linked (7076) and goat anti-rabbit IgG HRP-linked (7074) antibodies were from Cell Signaling.
Goat anti rabbit igg hrp linked 7074
Manufactured by Cell Signaling Technology
Goat anti-rabbit IgG HRP-linked (7074) is a secondary antibody that binds to rabbit primary antibodies. The antibody is conjugated with horseradish peroxidase (HRP), which allows for colorimetric or chemiluminescent detection in various immunoassay applications.
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2 protocols using goat anti rabbit igg hrp linked 7074
1
Immunofluorescence and Western Blotting Antibodies
2
Antibody Sources and Characterization
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Antibodies were obtained from Santa Cruz Biotechnology (Tubulin sc-5286, p27 Antibody (F-8) sc-1641), Bethyl Laboratories (CNOT3 A302-156A, CNOT2 A302-562A, RNF219 A302-540A (RNF219-C), RPL7a A300-749A), Proteintech (CNOT1 14276-1-AP), Covance (anti-HA.11, MMS-101P) and Abcam (GAPDH ab9485). FLAG-M2 agarose (F2426 or A2220-5ML) and FLAG peptide (F3290-4MG) were purchased from Sigma-Aldrich. RNF219 specific antibodies were produced using an internal (RNF219-A) or C-terminal (RNF219-B) peptide by Abnova (Taiwan). Secondary antibodies were from Cell Signaling Technology (goat anti-mouse IgG HRP-linked, #7076 and goat anti-rabbit IgG HRP-linked, #7074).
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