Advanced model 3300 micro osmometer

Manufactured by Advanced Instruments

Sourced in United States

The Advanced® Model 3300 micro-osmometer is a laboratory instrument designed to measure the osmolality of small sample volumes. It utilizes the freezing point depression method to determine the osmolality of biological and non-biological fluids.

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Lab products found in correlation

Combur10test ux test strips, by Roche (1 mentions) Metabolic cage, by Tecniplast (1 mentions) Stat profile phox ultra analyzer, by Nova Biomedical (1 mentions) Au5800 chemistry analyzer, by Beckman Coulter (1 mentions) Aution max ax 4030, by Arkray (1 mentions)

Spelling variants of this product

Micro Osmometer Model 3300 (8 citations) Micro-osmometer (8 citations) Model 3300 (8 citations) Advanced Micro Osmometer 3300 (6 citations) Micro-Osmometer 3300 (4 citations) Advanced Micro Osmometer (4 citations)

6 protocols using advanced model 3300 micro osmometer

Urine Analysis in Rat Toxicity Studies

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In the case of the two 90-day feeding trials, an analysis of urine of 16 males and 16 females per group was performed at the end of the study. In the combined chronic toxicity/carcinogenicity study, an analysis of urine of 20 male and 20 female rats per group was performed at months 3, 6, and 12; at month 24, urine samples from all surviving animals were analysed.
Urine was collected from each individual rat in metabolic cages for 16 h. The parameters total protein, glucose, ketone, leukocyte number, erythrocyte number, bilirubin, urobilinogen, nitrate, and pH were analyzed with Combur¹⁰Test^® UX test strips (Roche Diagnostics, Mannheim, Germany) and semi-quantitatively evaluated by reflectance photometry with a Urilux S analyzer (Roche Diagnostics). Osmolarity was measured with the Advanced^® Model 3300 micro-osmometer from Advanced Instruments (Norwood, MA, USA).

Steinberg P., van der Voet H., Goedhart P.W., Kleter G., Kok E.J., Pla M., Nadal A., Zeljenková D., Aláčová R., Babincová J., Rollerová E., Jaďuďová S., Kebis A., Szabova E., Tulinská J., Líšková A., Takácsová M., Mikušová M.L., Krivošíková Z., Spök A., Racovita M., de Vriend H., Alison R., Alison C., Baumgärtner W., Becker K., Lempp C., Schmicke M., Schrenk D., Pöting A., Schiemann J, & Wilhelm R. (2019). Lack of adverse effects in subchronic and chronic toxicity/carcinogenicity studies on the glyphosate-resistant genetically modified maize NK603 in Wistar Han RCC rats. Archives of Toxicology, 93(4), 1095-1139.

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Metabolic Cage Urine Analysis

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The mice were placed in metabolic cages (Techniplast, Exton, PA) with free access to food and water. After a period of 3 days of acclimatization, urine samples were collected for 24 h. We measured urine volume and urine osmolality by freezing point depression with an Advanced model 3300 micro-osmometer (Advanced Instruments Inc., Norwood, MA). We also measured urinary Na⁺, K⁺, Cl⁻, Mg²⁺, Ca²⁺, and creatinine with a Stat Profile pHOx Ultra Analyzer (Nova Biomedical, Waltham, MA). Then mice were restricted from access to water for 24 h. During this period, urine was collected to measure the parameters again. Then we let the mice recover for 3 days, placed them on low Na⁺ diet, and collected urine to measure the parameters again. Serum Na⁺ and K⁺ were measured in a NOVA 1+ analyzer (Nova Biomedical).

Caceres P.S., Mendez M., Haque M.Z, & Ortiz P.A. (2016). Vesicle-associated Membrane Protein 3 (VAMP3) Mediates Constitutive Trafficking of the Renal Co-transporter NKCC2 in Thick Ascending Limbs: ROLE IN RENAL FUNCTION AND BLOOD PRESSURE. The Journal of Biological Chemistry, 291(42), 22063-22073.

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Plasma Electrolyte Analysis Protocol

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The blood samples were spun at 5000 g for 5 min in a Jouan A14 centrifuge (Thermo Fisher Scientific, MA, USA). The plasma was removed and immediately placed on ice. Plasma osmolality was determined using an Advanced^® Model 3300 micro-osmometer (Advanced Instruments, MA, USA). Plasma [Na⁺], [K⁺] and [Ca²⁺] were determined with an Eppendorf Elex 6361 flame photometer (Eppendorf, Hamburg, Germany), zeroed with a Lithium diluent blank and calibrated with a flame photometer serum standard (Eppendorf, Hamburg, Germany), using plasma diluted in 5 mM Lithium diluent (1:50).

Brijs J., Sandblom E., Sundh H., Gräns A., Hinchcliffe J., Ekström A., Sundell K., Olsson C., Axelsson M, & Pichaud N. (2017). Increased mitochondrial coupling and anaerobic capacity minimizes aerobic costs of trout in the sea. Scientific Reports, 7, 45778.

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Osmolality Measurement Methodology

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Osmolality readings were obtained on an Advanced Instruments Advanced™ Micro Osmometer Model 3300 (Advanced Instruments, Inc. Norwood, MA, USA). A saline solution (0.9%) (AddiPak, Teleflex Inc., Wayne, PA, USA) was run as a positive control. Each sample was calculated as the mean from back-to-back triplicate measurements. When replicate batches were measured, data are reported as mean +/− SEM.

Wallach J., Gamrat J., Jauhola-Straight R., Becker J, & Eckrich T. (2022). Three Birds, One Excipient: Development of an Improved pH, Isotonic, and Buffered Ketamine Formulation for Subcutaneous Injection. Pharmaceutics, 14(3), 556.

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Biomarkers of Kidney, Inflammation, and Muscle Damage in Athletes

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Data on serum and urine biomarkers of kidney function (serum creatinine, urea, serum sodium, and urine test strip), inflammation (C-reactive protein), and muscle damage (creatine kinase) were collected 24 h pre-race, immediately post-race, and at 48 h post-race.
Fasting blood samples (3 × 10 ml) were obtained from the antecubital vein in EDTA vacutainers at 24 h pre-race, immediately post-race, and at 48 h post-race. The blood samples were centrifuged at 3,000 rpm at 4°C for 10 min in a bench-top centrifuge. The supernatant serum was aliquoted and stored on dry ice until all samples were frozen at −80°C in sealed Eppendorf tubes to avoid evaporation. Serum urea, creatinine, CRP, CK, and Na⁺ were determined using an AU-5800 Chemistry Analyzer (Beckman Coulter Inc., CA, United States). Serum osmolality was determined with an Advanced Micro-Osmometer, Model 3300 (Advanced Instruments Inc., MA, United States) using freezing point depression. Urine tests were measured with AUTION MAX AX-4030 (ARKRAY Inc., MN, United States) analyzer with Uriflet S 9UB strips.

Nescolarde L., Roca E., Bogónez-Franco P., Hernández-Hermoso J., Bayes-Genis A, & Ara J. (2020). Relationship Between Bioimpedance Vector Displacement and Renal Function After a Marathon in Non-elite Runners. Frontiers in Physiology, 11, 352.

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Osmolality Measurement of Lyophilized Formulations

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The osmolality of the formulations was measured using an Advanced^® Micro-Osmometer (Model 3300, Advanced Instruments Inc., Norwood, MA, USA) based upon the freezing point depression method. Calibration of the device was performed using Clinitrol™ 290 reference solution (Advanced Instruments Inc., Norwood, MA, USA). As the osmolality of some formulations was higher than the upper range value of 2000 mOsm/kg, all measured samples were diluted (1:1) with milliQ water and the result was multiplied by two. The measurements were conducted in triplicate (on 20-µL aliquots) and mean values were reported.
Following conditions were analyzed:

Reference: 0.5 M NaOAc pH 5 buffer

Basic condition: 0.5 M NaOAc pH 5 buffer + excipients lyophilization

Intermediate condition: 0.5 M NaOAc/10% Ethanol pH 5 buffer + excipients lyophilization

Final condition: 0.5 M NaOAc/10% Ethanol pH 5 buffer + excipients lyophilization + 5 mg VitC

Additionally, the final condition was tested in a concentrated and diluted form, simulating the 2.2 and 10 mL radiolabeling volume.

Baudhuin H., Cousaert J., Vanwolleghem P., Raes G., Caveliers V., Keyaerts M., Lahoutte T, & Xavier C. (2021). 68Ga-Labeling: Laying the Foundation for an Anti-Radiolytic Formulation for NOTA-sdAb PET Tracers. Pharmaceuticals, 14(5), 448.

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